Finasteride Reduces Microvessel Density and Expression of Vascular Endothelial Growth Factor in Renal Tissue of Diabetic Rats
| Autor: | Li-shun Wei, Dong-ling Jin, He-lin Tian, Ru-tong Zhao, Hai-ying Wu, Chao-xian Zhao, Zhong-xin Xu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Renal cortex Kidney Glomerulus urologic and male genital diseases Diabetes Mellitus Experimental chemistry.chemical_compound 5-alpha Reductase Inhibitors Internal medicine Diabetes mellitus Animals Humans Medicine Diabetic Nephropathies RNA Messenger Rats Wistar Kidney business.industry Finasteride General Medicine Hyperplasia medicine.disease Streptozotocin Rats Vascular endothelial growth factor Endocrinology medicine.anatomical_structure Gene Expression Regulation chemistry Microvessels Immunohistochemistry business Diabetic Angiopathies medicine.drug |
| Zdroj: | The American Journal of the Medical Sciences. 349:516-520 |
| ISSN: | 0002-9629 |
| DOI: | 10.1097/maj.0000000000000451 |
| Popis: | Vascular endothelial growth factor (VEGF) plays a critical role in the pathogenesis of diabetic microvascular complications. Finasteride has been confirmed to decrease VEGF expression in prostate and prostatic suburethral tissue resulting in limiting hematuria from human benign prostatic hyperplasia. The purpose of this study was to evaluate the effects of finasteride on microvessel density (MVD), VEGF protein and mRNA expressions in the renal tissue of diabetic rats.Diabetic rats induced by streptozotocin were intragastrically given finasteride at 30 mg/kg body weight once a day for 4 weeks. Histomorphologic changes in kidney were observed under light microscope. Immunohistochemistry for CD34 and VEGF on kidney sections was performed to assess MVD and VEGF protein expression in glomeruli of rats, respectively. The VEGF mRNA expression in the renal tissue was examined using reverse transcription polymerase chain reaction analysis.The glomerular tuft area, glomerular volume, MVD, VEGF protein expression in glomeruli and VEGF mRNA expression in the renal cortex tissue were significantly increased in diabetic rats and finasteride-treated rats when compared with controls (P0.01, P0.05). When compared with diabetic rats, the glomerular tuft area, glomerular volume, MVD, VEGF protein expression in glomeruli and VEGF mRNA expression in the renal cortex tissue of finasteride-treated rats were significantly decreased (P0.05, P0.01).Finasteride reduces the VEGF expression and decreases the MVD in the renal tissue of diabetic rats, suggesting the therapeutic potential of finasteride on diabetic microvascular complications. |
| Databáze: | OpenAIRE |
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