Gonadotropin purification from horse serum applying magnetic beads
| Autor: | Andrea Preußer-Kunze, Christine Müller, Kerstin Wagner, Matthias Franzreb |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chromatography
Metaphosphoric acid Chemistry medicine.drug_class General Medicine Fractionation Chorionic Gonadotropin Applied Microbiology and Biotechnology Absorption Magnetics medicine Animals Molecular Medicine Horses Bioprocess Gonadotropin Horse serum Equine chorionic gonadotropin Dialysis (biochemistry) Ethanol precipitation |
| Zdroj: | Biotechnology Journal. 6:392-395 |
| ISSN: | 1860-6768 |
| Popis: | The glycoprotein hormone equine chorionic gonadotropin (eCG) is a commercial product used in animal breeding as well as in veterinary medicine. The current state of the art for the purification of eCG from serum is pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Two simplified processes, including the use of magnetic microsorbents for the purification of eCG have been developed. The processes reduce or even omit the use of organic solvents and the required solid-liquid separation steps, thus making them potential candidates for a first commercial application of magnetic beads in bioprocessing. |
| Databáze: | OpenAIRE |
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