Non-invasive real-time biopsy of intracranial lesions using short time expanded circulating tumor cells on glass slide: report of two cases

Autor: A. M. Lavecchia, Angelo Lavano, A. Della Torre, Valentina Trunzo, Natalia Malara, Giusy Guzzi, Chiara Mignogna, A. Di Vito, Micaela Gliozzi, Vincenzo Mollace, Volpentesta G, Caterina Camastra, Giuseppe Donato, C. Ceccotti
Rok vydání: 2015
Předmět:
0301 basic medicine
Adult
Male
medicine.medical_specialty
Pathology
Neurology
Biopsy
Cytodiagnosis
Clinical Neurology
Contrast Media
Case Report
Astrocytoma
Intracranial tumor
Lesion
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
Medicine
Humans
Medical diagnosis
Cells
Cultured
Aged
Retrospective Studies
medicine.diagnostic_test
business.industry
Brain Neoplasms
Circulating tumor cells
Magnetic resonance imaging
Neoplasms
Second Primary
General Medicine
Neoplastic Cells
Circulating
Magnetic Resonance Imaging
Short-term expansion
030104 developmental biology
030220 oncology & carcinogenesis
Positron-Emission Tomography
Intracranial lesions
Female
Neurology (clinical)
Neurosurgery
Radiology
Lymphoma
Large B-Cell
Diffuse
medicine.symptom
business
Glioblastoma
Tomography
X-Ray Computed
Zdroj: BMC Neurology
ISSN: 1471-2377
Popis: Background Circulating Tumor Cells (CTCs) are promising biomarkers for monitoring solid cancer and were used to monitor brain tumors. Here we report two cases in which, for the first time, CTCs were used in cytological diagnostic evaluation to discriminate a space-occupying lesion of the brain. Case presentation Two cases of focal intracranial lesions, unclassified for diagnosis, untreated and apparently symptomatic, were examined after high-contrast resolution Magnetic Resonance Imaging and/or Computed Tomography scans. CTCs were seeded on chamber slides and short-time expanded under the optimized conditions as we previously reported. The first case was a focal lesion localized in the parietal-occipital area in a 67-year-old woman. The second case was a 31-year-old man with an expansive intracerebral lesion localized in the left peri-trigonal area. Both patients underwent excisional biopsy. Histopathological evaluation of the biopsy confirmed the previous cytological diagnoses, and the analysis of the clinical outcomes retrospectively validated both diagnoses. Conclusions The cases here reported illustrate the potential for using expanded CTCs as non-invasive, real-time biopsy. Moreover, non-invasive real-time biopsy can represent an alternative diagnostic tool to be used when a functional area of the brain is at risk of injury from excisional biopsy procedures.
Databáze: OpenAIRE
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