Microvessel Density Evaluation of the Effect of Enamel Matrix Derivative on Soft Tissue After Implant Placement: A Preliminary Study
| Autor: | Daiane Cristina Peruzzo, James Carlos Nery, George Furtado Guimarães, Vera Cavalcanti de Araújo, Andresa Borges Soares |
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| Rok vydání: | 2015 |
| Předmět: |
Angiogenesis
Biopsy Gingiva Dentistry Neovascularization Physiologic Surgical Flaps Neovascularization Immunoenzyme Techniques 03 medical and health sciences 0302 clinical medicine Dental Enamel Proteins Enamel matrix derivative medicine Maxilla Humans Wound Healing medicine.diagnostic_test business.industry Dental Implantation Endosseous Soft tissue Histology 030206 dentistry Treatment Outcome 030220 oncology & carcinogenesis Microvessels Periodontics Angiogenesis Inducing Agents Implant Oral Surgery medicine.symptom business Wound healing |
| Zdroj: | The International journal of periodonticsrestorative dentistry. 35(5) |
| ISSN: | 1945-3388 |
| Popis: | Enamel matrix derivative (EMD) is commonly used in periodontal therapy and has been used successfully for periodontal regeneration. In addition, this material has a possible angiogenic effect that has been associated with enhanced wound healing. The aim of this study was to evaluate the effect of EMD on microvessel density (angiogenesis) on the soft tissues surrounding newly placed implants after 14 days. Five patients were selected, each requiring at least one implant on each side of the maxilla, in a split-mouth experimental design. The implants were placed in a two-stage procedure. Each side was then randomized as test or control. On the test side, 0.1 mL of EMD was topically applied to the soft tissues surrounding the implants, while the control side did not receive any treatment. Second-stage surgery was performed after 14 days. A 6-mm punch biopsy was performed for each implant, with the samples subsequently prepared for histology and immunohistochemistry. Quantitative vascularization analysis was performed, which involved counting three areas or "hotspots" containing vessels strongly positive for CD34 and CD105, a pan-endothelial and new vessel marker, respectively. There was no significant difference between test and control groups when evaluating the formation of new blood vessels. The total number of blood vessels, however, was significantly higher in the group treated with EMD (test group). Within the limits of the present study, it can be concluded that topical application of EMD on the soft tissues surrounding newly placed implants resulted in an increased number of blood vessels at 14 days, suggesting that EMD may play a beneficial role in this aspect of wound healing. |
| Databáze: | OpenAIRE |
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