Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress
| Autor: | Heuy-Ling Chu, Charng-Cherng Chyau, Zong-Tsi Chen, Chin-Chen Chu, Pin-Der Duh |
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| Rok vydání: | 2012 |
| Předmět: |
Antioxidant
medicine.medical_treatment Protective Agents medicine.disease_cause Nobiletin Analytical Chemistry chemistry.chemical_compound Hesperidin medicine TBARS Humans Chromatography High Pressure Liquid Membrane Potential Mitochondrial Caspase 3 Plant Extracts Hesperetin Hep G2 Cells General Medicine Glutathione Oxidative Stress chemistry Biochemistry Fruit Oxidative stress Citrus × sinensis Citrus sinensis Food Science |
| Zdroj: | Food Chemistry. 135:2119-2127 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2012.07.041 |
| Popis: | Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress were investigated. According to HPLC-DAD and HPLC-MS/MS analysis, hesperidin (HD), hesperetin (HT), nobiletin (NT), and tangeretin (TT) were present in water extracts of sweet orange peel (WESP). The cytotoxic effect in 0.2mM t-BHP-induced HepG2 cells was inhibited by WESP and their bioactive compounds. The protective effect of WESP and their bioactive compounds in 0.2mM t-BHP-induced HepG2 cells may be associated with positive regulation of GSH levels and antioxidant enzymes, decrease in ROS formation and TBARS generation, increase in the mitochondria membrane potential and Bcl-2/Bax ratio, as well as decrease in caspase-3 activation. Overall, WESP displayed a significant cytoprotective effect against oxidative stress, which may be most likely because of the phenolics-related bioactive compounds in WESP, leading to maintenance of the normal redox status of cells. |
| Databáze: | OpenAIRE |
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