Effect of bax, bcl-2 and bcl-xL on regulating apoptosis in tissues of normal liver and hepatocellular carcinoma
| Autor: | Xiaodong Shao, Li-Nan Ren, Di Wang, Jian-Hua Xu, Hongyu Li, Min-Pei Liu, Xiaozhong Guo, Jia-Jun Zhao |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Liver Cancer Pathology medicine.medical_specialty Carcinoma Hepatocellular bcl-X Protein Gene Expression Apoptosis Bcl-xL Bcl-2-associated X protein Proto-Oncogene Proteins Gene expression medicine Humans RNA Messenger RNA Neoplasm Northern blot Gene Aged bcl-2-Associated X Protein Messenger RNA biology Liver Neoplasms Gastroenterology General Medicine Middle Aged medicine.disease digestive system diseases Genes bcl-2 Liver Proto-Oncogene Proteins c-bcl-2 Case-Control Studies Hepatocellular carcinoma Cancer research biology.protein Female |
| Zdroj: | World Journal of Gastroenterology. 8:1059 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v8.i6.1059 |
| Popis: | AIM: To investigate the expression of bax, bcl-2 and bcl-xL mRNA in the tissues of normal liver and hepatocellular carcinoma (HCC), and analyze the relationship between the expression of bax, bcl-2 and bcl-xL mRNA and clinical parameters of HCC patients. METHODS: The expression of bax, bcl-2 and bcl-xL mRNA of normal liver and HCC was measured by Northern blot. Statistical analyses were made by t test and correlation analysis. RESULTS: A very low mRNA level was indicated at bax, bcl-2 and bcl-xL in the HCC tissues in contrast to the tissues of normal liver by Northern blot analysis. The analyses of mRNA level revealed that HCC tissues exhibited a mean 7.6-fold decrease in bax, 4.2-fold in bcl-2 and 3.5-fold in bcl-xL in comparison with normal control tissues, respectively. Positive correlation was found between bax and bcl-xL (r = 0.7061,P < 0.01). There was no significance between the mRNA expression of these three genes and age, gender, tumor differentiation and tumor stage of HCC patients . CONCLUSION: The results are consistent with the fact that apoptosis rarely occurs in normal livers but increases in HCC, indicating that bcl-2 and bcl-xL may play a very important role in regulating the apoptosis of normal liver and HCC. |
| Databáze: | OpenAIRE |
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