The thermal behaviour of enzyme activity: implications for biotechnology

Autor:	Michelle E. Peterson, Michael J. Danson, Roy M. Daniel, Robert Eisenthal
Rok vydání:	2006
Předmět:	chemistry.chemical_classification biology business.industry Temperature Bioengineering Protein engineering Protein Engineering Enzyme assay Enzymes Biotechnology Bioreactors Enzyme Models Chemical chemistry Enzyme reactor Enzyme Stability biology.protein business
Zdroj:	Trends in Biotechnology. 24:289-292
ISSN:	0167-7799
DOI:	10.1016/j.tibtech.2006.05.004
Popis:	The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e477573d1972db0fd84966be8830b7da https://doi.org/10.1016/j.tibtech.2006.05.004 Zobrazit plný text záznamu Full Text from ScienceDirect