Monitoring Photosynthesis in Individual Cells of Synechocystis sp. PCC 6803 on a Picosecond Timescale
| Autor: | Sashka Krumova, G. Ajlani, Zoltán Gombos, Sergey P. Laptenok, H. van Amerongen, Bettina Ughy, Jan Willem Borst |
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| Přispěvatelé: | Système membranaires, photobiologie, stress et détoxication (SMPSD), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
spectroscopy Time Factors Photosystem II Kinetics Spectroscopy Imaging and Other Techniques Biophysics Biochemie synechococcus 6301 macromolecular substances Biology Photosynthesis Photosystem I Photochemistry 01 natural sciences Biochemistry intact photosystem-ii 03 medical and health sciences excitation-energy transfer Phycobilisomes chlorophyll time 030304 developmental biology 0303 health sciences Photosystem I Protein Complex Synechocystis Photosystem II Protein Complex food and beverages [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology thylakoid membrane biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology phycobilisome fluorescence emission-spectra Biofysica Microscopy Fluorescence kinetics Thylakoid Picosecond Mutation Phycobilisome 010606 plant biology & botany |
| Zdroj: | Biophysical Journal Biophysical Journal, 2010, 99 (6), pp.2006-2015. ⟨10.1016/j.bpj.2010.07.015⟩ Biophysical Journal 99 (2010) 6 Biophysical Journal, 99(6), 2006-2015 |
| ISSN: | 0006-3495 1542-0086 |
| DOI: | 10.1016/j.bpj.2010.07.015 |
| Popis: | Picosecond fluorescence kinetics of wild-type (WT) and mutant cells of Synechocystis sp. PCC 6803, were studied at the ensemble level with a streak-camera and at the cell level using fluorescence-lifetime-imaging microscopy (FLIM). The FLIM measurements are in good agreement with the ensemble measurements, but they (can) unveil variations between and within cells. The BE mutant cells, devoid of photosystem II (PSII) and of the light-harvesting phycobilisomes, allowed the study of photosystem I (PSI) in vivo for the first time, and the observed 6-ps equilibration process and 25-ps trapping process are the same as found previously for isolated PSI. No major differences are detected between different cells. The PAL mutant cells, devoid of phycobilisomes, show four lifetimes: similar to 20 Ps (PSI and PSII), similar to 80 ps, similar to 440 ps, and 2.8 ns (all due to PSII), but not all cells are identical and variations in the kinetics are traced back to differences in the PSI/PSII ratio. Finally, FLIM measurements on WT cells reveal that in some cells or parts of cells, phycobilisomes are disconnected from PSI/PSII. It is argued that the FLIM setup used can become instrumental in unraveling photosynthetic regulation mechanisms in the future. |
| Databáze: | OpenAIRE |
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