Simultaneous Determination of Eight Components in Leonuri Herba from Different Habitats by HPLC-DAD
| Autor: | Fang Wang, Huailei Yang, Yang Xu, Huiwei Bao, Jihong Chi |
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| Rok vydání: | 2018 |
| Předmět: |
Chromatography
010405 organic chemistry Reproducibility of Results General Medicine 01 natural sciences 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Ethanol extracts Leonurus chemistry Drug Stability Limit of Detection Gradient elution Methanol Phosphoric acid Hplc dad Chromatography High Pressure Liquid Ecosystem Drugs Chinese Herbal |
| Zdroj: | Journal of chromatographic science. 58(5) |
| ISSN: | 1945-239X |
| Popis: | A HPLC-DAD method was established for the simultaneous determination of eight components in Leonuri Herba from different habitats, as well as providing methodological reference for quality control of Leonuri Herba. In this study, absolute ethanol extracts of the medicinal material were considered as the sample solutions to be analyzed. Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used for determination maintained at the temperature of 30°C. Gradient elution was performed with a mobile phase of methanol (B)-0.1% phosphoric acid solution (A) at the flow rate of 1.0 mL·min−1. The analysis was carried out at the wavelength of 225, 280 and 320 nm. The measured eight components showed good linear relationships within their own concentration range. Average recoveries ranged from 98.83 to 100.36% with RSDs of 1.14~1.97%. The proposed method was found to be simple, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Leonuri Herba from different habitats. |
| Databáze: | OpenAIRE |
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