Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
| Autor: | Zdzisław Targoński, Piotr Jarocki, Michał Dworniczak, Jarosław Pawelec, Marcin Podleśny, Mariusz Krawczyk, Oleksandr Kholiavskyi, Elwira Komoń-Janczara, Agnieszka Glibowska |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Locus (genetics) Microbiology law.invention 03 medical and health sciences Probiotic Lactobacillus rhamnosus law Virology lcsh:RC799-869 ORFS Gene Prophage Whole genome sequencing Lactobacillus rhamnosus Pen biology Probiotics Gastroenterology biology.organism_classification CRISPR–Cas locus Genome Report 030104 developmental biology Infectious Diseases Genome sequence lcsh:Diseases of the digestive system. Gastroenterology Parasitology GC-content |
| Zdroj: | Gut Pathogens Gut Pathogens, Vol 10, Iss 1, Pp 1-6 (2018) |
| ISSN: | 1757-4749 |
| Popis: | Background Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. Results Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified. Conclusions Genomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain. Electronic supplementary material The online version of this article (10.1186/s13099-018-0235-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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