Confirmation of hepatitis C virus infection by new four-antigen recombinant immunoblot assay
| Autor: | W. Schaasberg, D. Mulder-Folkerts, H. W. Reesink, A. Leentvaar-Kuypers, Michael Houghton, I.N. Winkel, C. L. Van Der Poel, R. Di Nello, P.J. Exel-Oehlers, A J Weiner, H. T. M. Cuypers, J. J. P. Van Boven, Stella Quan, Alan Polito, P. N. Lelie |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Hepatitis C virus
Immunoblotting Molecular Sequence Data Hepacivirus Biology medicine.disease_cause Polymerase Chain Reaction law.invention Antigen law medicine Humans Immunoblot Assay Hepatitis Antibodies Prospective Studies Polymerase chain reaction Hepatitis Chronic Hepatitis Base Sequence Plasma samples General Medicine medicine.disease Hepatitis C Virology Evaluation Studies as Topic Immunology Recombinant DNA Viral disease Follow-Up Studies |
| Zdroj: | Lancet, 337(8737), 317-319. Elsevier Limited |
| ISSN: | 0140-6736 |
| Popis: | A new four-antigen recombinant immunoblot assay (4-RIBA) for confirmation of hepatitis C virus (HCV) C-100 enzyme-linked immunosorbent assay (ELISA) reactivity was tested in stored serum samples (1984-86) of blood donors and recipients and compared with results from polymerase chain reaction (PCR) analysis of fresh (1990) plasma samples in donors and recipients from the original study. Of 37 HCV C-100 ELISA-positive blood products, 8 were 4-RIBA positive, of which 7 were implicated in post-transfusion non-A, non-B hepatitis (PT-NANBH) and/or PCR confirmed recipient HCV infection. Of 9 recipients with PT-NANBH, 8 were reactive in 4-RI BA (6 positive and 2 indeterminate). With fresh plasma samples, 3 donors and 6 recipients who were 4-RIBA positive were also PCR positive. 4 4-RIBA indeterminate and 78 4-RIBA negative samples of donors and recipients were PCR negative. Of 64-RIBA positive recipients, 5 were PCR positive four to six years later. 1·6% of the 383 recipients became chronically infected with HCV. The new 4-RIBA represents a candidate confirmation test to discriminate between infective and non-infective HCV C-100 ELISA-positive blood donors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|