A Review on Computational Approaches for Analyzing Hydrogen- Deuterium (H/D) Exchange of Proteins
| Autor: | Thirunavukkarasu Sivaraman |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Physics
0303 health sciences Hydrogen 010401 analytical chemistry Deuterium Exchange Measurement Proteins chemistry.chemical_element General Medicine Mass spectrometry 01 natural sciences Biochemistry 0104 chemical sciences 03 medical and health sciences Deuterium chemistry Structural Biology Chemical physics Native state Spectroscopy Nuclear Magnetic Resonance Biomolecular 030304 developmental biology |
| Zdroj: | Protein & Peptide Letters. 28:372-381 |
| ISSN: | 0929-8665 |
| DOI: | 10.2174/0929866527666201002145859 |
| Popis: | Native state Hydrogen-Deuterium (H/D) exchange method has been used to study the structures and the unfolding pathways for quite a number of proteins. The H/D exchange method is generally monitored using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) techniques. NMR-assisted H/D exchange methods primarily monitor the residue level fluctuation of proteins, whereas MS-assisted H/D exchange methods analyze multifold ensemble conformations of proteins. In this connection, quite a large number of computational tools and algorithms have been developed for processing and analyzing huge amount of the H/D exchange data generated from these techniques. In this review, most of the freely available computational tools associated with the H/D exchange of proteins have been comprehensively reviewed and scopes to improve/ develop novel computational approaches for analyzing the H/D exchange data of proteins have also been brought into fore. |
| Databáze: | OpenAIRE |
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