Molecular cloning and characterization of an allatostatin-like receptor in the cockroach Diploptera punctata
| Autor: | Stephen S. Tobe, Jinrui Zhang, William G. Bendena, Panida Lungchukiet, B. Cameron Donly |
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| Rok vydání: | 2007 |
| Předmět: |
Receptors
Neuropeptide animal structures DNA Complementary Physiology ved/biology.organism_classification_rank.species Molecular Sequence Data Gene Expression Cockroaches Biology Biochemistry Cellular and Molecular Neuroscience Endocrinology Oogenesis Corpora Allata Complementary DNA biology.animal Animals Amino Acid Sequence Cloning Molecular Receptor Phylogeny G protein-coupled receptor RNA Double-Stranded Cockroach Base Sequence Sequence Homology Amino Acid ved/biology Reproduction Diploptera punctata Neuropeptides Allatostatin Sequence Analysis DNA Molecular biology Cell biology Juvenile Hormones RNA silencing Juvenile hormone Female RNA Interference |
| Zdroj: | Peptides. 29(2) |
| ISSN: | 0196-9781 |
| Popis: | Two Drosophila receptors (AlstR/DAR-1 and DAR-2) with sequence similarity to mammalian galanin receptors have been previously identified. These receptors have been shown to form specific interactions with neuropeptides that resemble cockroach allatostatins (ASTs), which have a characteristic Tyr/Phe-Xaa-Phe-Gly-Leu-NH 2 carboxyl-terminus. We hypothesized that similar allatostatin receptors exist in the cockroach Diploptera punctata that may regulate the numerous effects that this family of peptides exerts on a range of target tissues. The polymerase chain reaction (PCR) was used, with primer design based on the Drosophila allatostatin receptor (AlstR). Using these primers, a putative allatostatin-like receptor cDNA was isolated from a λZAP-cDNA library prepared from the corpora allata of the D. punctata . As an approach to testing the function of this receptor in vivo , the technique of double-stranded RNA (dsRNA) gene interference was tested. Initial experiments suggest that the putative inhibition of receptor RNA expression may increase juvenile hormone (JH) production. |
| Databáze: | OpenAIRE |
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