The pro-form of BMP-2 exhibits a delayed and reduced activity when compared to mature BMP-2
| Autor: | Bernhard Frerich, Katrin Bigl, Elisabeth Schwarz, Silvio Erler, Sabrina von Einem |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Time Factors
medicine.medical_treatment Cellular differentiation Clinical Biochemistry Osteocalcin Bone Morphogenetic Protein 2 Down-Regulation Biology Bone morphogenetic protein 2 Cell Line Mice Endocrinology medicine Animals Secretion Protein Precursors Osteoblasts Growth factor Cell Differentiation Cell Biology Alkaline Phosphatase Cell biology Biochemistry Gene Expression Regulation Cell culture Alkaline phosphatase Signal transduction C2C12 Signal Transduction Transcription Factors |
| Zdroj: | Growth factors (Chur, Switzerland). 29(2-3) |
| ISSN: | 1029-2292 |
| Popis: | Pro-forms of growth factors receive increasing attention since it was shown that they can affect the function(s) of the mature proteins. Here, we extend our previous investigations on the biological function of the pro-form of bone morphogenetic protein-2 (BMP-2). We demonstrate that proBMP-2, upon prolonged incubation with C2C12 cells, induces alkaline phosphatase, a marker enzyme for osteoblastic differentiation. Expression studies with three different bone marker transcripts reveal that proBMP-2 induces bone-specific transcripts, however, to a smaller extent than the mature growth factor. To resolve this finding at the protein level, the fate of proBMP-2 and BMP-2 was studied in cell culture. We demonstrate that both proteins become internalized, and proBMP-2 is processed to mature BMP-2 within the cells. The data presented here suggest that proBMP-2 elicits biological functions as mature BMP-2 at a delayed and reduced level, which might depend on intracellular cleavage and subsequent secretion as mature BMP-2. |
| Databáze: | OpenAIRE |
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