In vitro Susceptibility of Madurella mycetomatis to the Extracts of Anogeissus leiocarpus Leaves

Autor: Elsiddig, Ikram Mohamed Eltayeb, Muddather, Abdel Khalig, Ali, Hiba Abdel Rahman, Ayoub, Saad Mohamed Hussein
Jazyk: angličtina
Rok vydání: 2015
Předmět:
DOI: 10.5281/zenodo.1110211
Popis: Anogeissus leiocarpus (Combretaceae) is well known for its medicinal uses in African traditional medicine, for treating many human diseases mainly skin diseases and infections. Mycetoma disease is a fungal and/ or bacterial skininfection, mainly cause by Madurella mycetomatis fungus. This study was carried out in vitro to investigate the antifungal activity of Anogeissus leiocarpus leaf extracts against the isolated pathogenic Madurella mycetomatis, by using the NCCLS modified method compared to Ketoconazole standard drug, and MTT assay. The bioactive fraction was subjected to chemical analysis implementing different chromatographic analytical methods (TLC, HPLC, and LC-MS/MS). The results showed significance antifungal activity of A. leiocarpus leaf extracts against the isolated pathogenic M. mycetomatis, compared to negative and positive controls. The chloroform fraction showed the highest antifungal activity. The chromatographic analysis of the chloroform fraction with the highest activity showed the presence of important bioactive compounds such as ellagic and flavellagic acids derivatives, flavonoids and stilbenoid, which are well known for their antifungal activity.
{"references":["Agaie, B.M.; Onyeyili, P.A.; Muhammad, B.Y. and Landan, M.J., 2007.\nSome Toxic Effects of Aqueous Leaf Extract of Anogeissus leiocarpus\nin rats. Journal of Pharmacology and Toxicology, 2(4): 396-401.","Adejumobi, J.A.; Ogundiya, M. O.; Kolapo, A.andkunade, M.B., 2008.\nPhytochemical composition and in vitro antimicrobial activity of\nAnogeissus leiocarpus on some common oral pathogens. Journal of\nMedicinal Plants Research, 2(8):193-196.","Okpekon, T.; Yolou, S.; Gleye, C.; Roblot, F.; Loiseau, P.; Bories, C.;\nGrellier, P.; Frappier F.; Laurens, A. and Hocquemiller, R., 2004.\nAntiparasitic activities of medicinal plants used in Ivory Coast. Journal\nof Ethnopharmacology, 90: 91-97.","Agaie, B. M. and Onyeyili, P. A., 2007. Anthelmintic activity of the\ncrude aqueous leaf extracts of Anogeissus leiocarpus in sheep. African\nJournal of Biotechnology, 6(13):1511-1515.","Adeleye, I.A.; Ogunniyi, A.A. and Omonigbehin, E. A., 2003.\nAntimicrobial activity of some local herbs on common skin pathogens.\nBioscience Research Communication, 15(3): 231-236.","Vonthron-Sénécheau, C.; Weniger, B.; Ouattara, M.; Tra Bi, F.;\nKamenan, A.; Lobstein, A.; Brun, R. and Anton, R., 2003. In vitro\nantiplasmodial activity and cytotoxicity of ethnobotanically selected\nIvorian plants. Journal of Ethnopharmacology, 87: 221-225.","Mustofa, V. A.; Benoˆıt-Vical, F.; Pellissier, Y.; Kone-Bamba, D. and\nMallié, M., 2000. Antiplasmodial activity of plants extracts used in West\nAfrican traditional Medicine. Journal of Ethnopharmacology, 73: 145-\n151.","Chaabi, M.; Benayache, S.; Vonthron-Sénécheau, C.; Weniger, B.;\nAnton, R. andLobstein, A., 2006. Antiprotozoal activity of saponins\nfrom Anogeissus leiocarpus (Combretaceae). Planta Med., 72: 7.","Almagboul, A. Z.; Basher, A. and Salih, A.K. M., 1988. Antimicrobial\nactivity of certain Sudanese plants used in folkloric medicine. Screening\nfor antifungal activity. Fitoterapia, 59: 393-396.\n[10] Mann, A.; Amupitan, J.O.; Oyewale, A.O.; Okogun, J.I.; Ibrahim, K.;\nOladosu, P.; Lawson, L.; Olajide, I. and Nnamdi, A., 2008. Evaluation\nof in vitro antimycobacterial activity of Nigerian plants used for\ntreatment of respiratory diseases. African Journal of Biotechnology,\n7(11): 1630-1636.\n[11] Ibrahim, M.B.; Owonubi, M.O.; Onaopo, J.A., 1997. Antibacterial effect\nof extract of leaf, stem and root bark of Anogeissus leiocarpus on some\nbacterial organisms. J. Pharm. Res. Dev., 2(1): 20-23.\n[12] Sanogo, R., 2005. Antifungal and Antioxidant Activities of 14 plants\nused in the treatment of sexually transmitted infections. Afr. J. Trad,\nComplem. Alter. Med.,2(2): 177- 205.\n[13] Batawila, K.; Kokou, K.; Koumaglo, K.; Gbéassor, M.; de Foucault,B.;\nBouchet, Ph. and Akpagana, K., 2005. Antifungal activities of five\nCombretaceae used in Togolese traditional medicine. Fitoterapia, 76:\n264-268.\n[14] Mann, A.; Yahaya Y.; Banso, A. and Ajayi, G.O., 2008.Phytochemical\nand antibacterial screening of Anogeissus leiocarpus against some\nmicroorganisms associated with infectious wounds. African Journal of\nMicrobiology Research, 2: 60-62.\n[15] Mann, A.; Banso, A. and Clifford, L.C., 2008. An antifungal property of\ncrude plant Extracts from Anogessius leiocarpus and Terminalia\navicennioides, Tanzania Journal of Health Researc.,10(1): 34-38.\n[16] Mann, A.; Amupitan, J.O.; Oyewale, A.O.; Okogun, J.I. and Ibrahim,\nK., 2009. Antibacterial activity of terpenoidal fractions from Anogeissus\nleiocarpus and Terminalia avicennioides against community acquired\ninfections. African Journal of Pharmacy and Pharmacology, 3(1): 22-25.\n[17] Mann, A.; Amupitan, J.O.; Oyewale, A.O.; Okogun, J.I. and Ibrahim,\nK., 2009. Chemistry of secondary metabolites and their antimicrobial\nactivity in the drug development process: A review of the genus\nAnogeissus. Medicinal Plants-International Journal of Phytomedicines\nand Related Industries, 1(2): 6.\n[18] Gumaa, S.A., 1994. The aetiology and epidemiology of mycetoma.\nSudan medical journal, 32(2): 14-22.\n[19] Mahgoub, E.S., 1994. Medical treatment of mycetoma. Sudan medical\njournal, 32(2): 88-97.\n[20] NCCLS, 2002. National Committee for Clinical Laboratory Standards.\n2002. Reference method for broth dilution antifungal susceptibility\ntesting of filamentous fungi. Approved standard NCCLS Document\nM38-A.9. National Committee for Clinical Laboratory Standards,\nWayne, USA.\n[21] Ahmed, A.O.; Van de Sande, W.W.; Van Vianen, W.; Belkum, Van\nAlex; Fahal, A.H.; Verbrug, H.A.; Irma, A. and Bakker-Woudenber,\nJ.M., 2004. In vitro Susceptibility of Madurella mycetomatis to\nItraconazole and Amphotericin B assed by a Modified NCCLS Method\nand a viability based 2,3-bis(2-methoxy 040nitro-5-sulfophenyl)-5-\n{(phenylamino) carbonyl}-2H-tetrazplium hydroxide (XTT) assay and a\nmodified NCCLS method. Journal of Antimicrobial Chemotherapy,\n48(7): 2742-2746. [22] Van de Sande, W.W.J.; Lujendijk, A. and Ahmed, A.O., 2005.Testing of\nthe in-vitro susceptibility of Madurella mycetomatis to six antifungal\nagents by using the sensititer system in comparison with viability based\n2,3-bis(2-methoxy040nitro-5-sulfophenyl)-5-{(phenylamino) carbonyl}-\n2H-tetrazplium hydroxide (XTT) assay and a modified NCCLS method.\nJournal of Antimicrobial Chemotherapy, 49:1364-1368.\n[23] Muraina, I.A.; Picard, J.A. and Eloff, J.N., 2009. Development of a\nreproducible method to determine minimum inhibitory concentration\n(MIC) of plant extract against a slow-growing mycoplasmas organism.\nPhytomedicine, 16(2-3): 262-264.\n[24] Kuo-Ching Wen, I-Chen Shih, Jhe-Cyuan Hu, Sue-Tsai Liao, Tsung-\nWei Su, and Hsiu-Mei Chiang, 2011. Inhibitory Effects of Terminalia\ncatappa on UVB-Induced Photodamage in Fibroblast Cell Line. Evid\nBased Complement Alternat Med.: 904532.\n[25] Ten-Ning, C.; Guan-Jhong, H.; Yu-Lin, H.; Shgh-Shgun, H.; Heng-Yua,\nC.; Yuan-Shium, C., 2009. Antioxidant and Antiproliferative Activities\nof Crossostephium chinenis. The American Journal of Chinese\nMedicine, 37(4): 797-814.\n[26] Eloff, J. N.; Katere, D. R.; McGaw, L.J., 2008. The biological activity\nand chemistry of the Southern African Combretaceae Journal of\nEthnopharmacology, 119: 686-699.\n[27] Reddy, K.K.; Rajadurai, S.; Sastry, K.N.S. and Nayudamma, Y., 1964.\nStudies on dhava tannins: Part I. The isolation and constitution of a\ngallotannin from dhava (Anogeissus latifolia). Australian Journal of\nChemistry, 17(2): 238-245.\n[28] Reddy, K.K.; Rajadurai, S. and Nayudamma,Y., 1965. Studies on Dhava\n(Anogeis suslatifolia) Tannins: Part III. Polyphenols of bark, sapwood\nand heartwood of Dhava. Indian. J. Chem., 27: 308-310.\n[29] Deshpande, V.H.; Patil, A.D.; Rama Roa, A.V. and Venkataraman.,\n1976. Methylellagic acid and methylflavellagic acid from Anogeis\nsuslatifolia bark, Ind. J. Chem., 14B: 641-643.\n[30] Govindarajan, R.; Vijayakumar, M.; Rao, Ch.V.;Shirwaikar, A.;Rawat,\nA.K.S.; Mehrotra, S. and Pushpangadan, P., 2004. Antioxidant potential\nof Anogeissus. Biol. Pharm. Bull., 27(8): 1266-1269.\n[31] Govindarajan, R.; Vijayakumar, M.; Rao, Ch.V.; Shirwaikar, A.;\nPushpangadan, P. andMehrotra, S., 2004. Healing potential of\nAnogeissus latifolia for dermal wounds in rats. Acta. Pharmaceutica.,\n54(4): 331-338.\n[32] Govindarajan, R.; Vijayakumar, M.; Shirwaikar, A.; Rawat A.K.S.;\nMehrotra, S.and Pushpangadan, P., 2005. Activity Guided Isolation of\nAntioxidant Tannoid Principles from Anogeissus latifolia. Natural\nProduct Sciences, 11(3):174-178.\n[33] Pradeep, H.A.; Khan, S.; Ravikumar, K.; Ahmed, M.F.; Rao, M.S.;\nKiranmai, M.; Reddy, D.S.; Ahamed, Sh.R.;and Ibrahim, M.,\n2009.Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in\nvivo studies. World. J. Gastroenterol., 15(38): 4816-4822.\n[34] Scalbert, A., 1991. Antimicrobial properties of tannins. Phytochemistry,\n30: Pp 3875-3883.\n[35] Cowan, M. M., 1999. Plant Products as Antimicrobial Agents. Clinical\nmicrobiology reviews, 12 (4): Pp 564-582.\n[36] Langcake P., 1981. Disease resistance of Vitis spp. and the production of\nthe stress metabolites resveratrol, e-viniferin, a-viniferin and\npterostilbene. Physiological Plant Pathology,18: 312–226.\n[37] Alessandro, M.; Marco, S.D.; Osti, F. and Cesari, A., 2000. Bioassays\non the activity of resveratrol, pterostilbene and phosphorous acid\ntowards fungal associated with esca of grape vine. Phytopathol.\nMediterr., 39: 357-365.\n[38] Alessandro, M.; Marco, S.D.; Osti, F. and Cesari, A., 2000. Bioassays\non the activity of resveratrol, pterostilbene and phosphorous acid\ntowards fungal associated with esca of grape vine. Phytopathol.\nMediterr., 39: 357-365.\n[39] Suh, N.; Paul, S.; Hao, X.; Simi, B.; Xiao, H.; Rimando, A.M. and\nReddy, B.S., 2007. Pterostilbene, an active constituent of Blueberries,\nsuppresses aberrant crypt foci formation in the azoxymethane-induced\ncolon carcinogenesis model in rats. Clin. Cancer Res., 13(1): 350-355.\n[40] Rimando, A.M. and Suh, N., 2008. Biological/Chemopreventive\nActivity of Stilbenes and their Effect on Colon Cancer. Planta Med.,\n74(13): 1635-1643."]}
Databáze: OpenAIRE
Externí odkaz: