N-acylhydrazone improves exercise intolerance in rats submitted to myocardial infarction by the recovery of calcium homeostasis in skeletal muscle
| Autor: | Gisele Zapata-Sudo, Eliezer J. Barreiro, Sharon S. Landgraf, Rodolfo C. Maia, Arthur Eugen Kümmerle, Jaqueline S da Silva, Sharlene L Pereira, Roberto T. Sudo, Celso Caruso-Neves, Carlos A. M. Fraga |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Myocardial Infarction Exercise intolerance Thiophenes General Biochemistry Genetics and Molecular Biology Sarcoplasmic Reticulum Calcium-Transporting ATPases Internal medicine Caffeine Physical Conditioning Animal medicine Animals Homeostasis Myocardial infarction General Pharmacology Toxicology and Pharmaceutics Rats Wistar Muscle Skeletal Calcium metabolism Exercise Tolerance business.industry Contractile response Hydrazones Skeletal muscle General Medicine medicine.disease Rats Artery ligation Disease Models Animal medicine.anatomical_structure Muscular fatigue Muscle Fatigue Cardiology Calcium medicine.symptom Once daily business Muscle Contraction |
| Zdroj: | Life sciences. 94(1) |
| ISSN: | 1879-0631 |
| Popis: | This work investigated the effects of 3,4-methylenedioxybenzoyl-2-thienylhydrazone (LASSBio-294) treatment on the contractile response of soleus (SOL) muscle from rats submitted to myocardial infarction (MI).Following coronary artery ligation, LASSBio-294 (2mg/kg, i.p.) or vehicle was administrated once daily for 4 weeks.The run time to fatigue for sham rats was 17.9 ±2.6 min, and it was reduced to 3.3 ± 0.8 min (P0.05) in MI rats. In MI rats treated with LASSBio-294, the time to fatigue was 15.1 ± 3.6 min. During the contractile test, SOL muscles from sham rats showed a response of 7.12 ± 0.54N/cm(2) at 60 Hz, which was decreased to 5.45 ± 0.49 N/cm(2) (P0.05) in MI rats. The contractility of SOL muscles from the MI-LASSBio-294 group was increased to 9.01 ± 0.65N/cm(2). At 16 mM caffeine, the contractility was reduced from 2.31 ± 0.33 to 1.60 ± 0.21 N/cm(2) (P0.05) in the MI group. In SOL muscles from MI-LASSBio-294 rats, the caffeine response was increased to 2.62 ± 0.33 N/cm(2). Moreover, SERCA2a expression in SOL muscles was decreased by 0.31-fold (31%) in the MI group compared to the Sham group (P0.05). In the MI-LASSBio-294 group, it was increased by 1.53-fold (153%) compared to the MI group (P0.05). Meanwhile, the nuclear density in SOL muscles was increased in the MI group compared to the Sham group. Treatment with LASSBio-294 prevented this enhancement of cellular infiltrate.LASSBio-294 treatment prevented the development of muscular fatigue and improved exercise intolerance in rats submitted to MI. |
| Databáze: | OpenAIRE |
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