Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
Autor: | José Gregorio Martínez, Jessica María Bedoya Vélez, Susana Ochoa Agudelo, Juliana Tobón Ospina |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Bioprospecting
biology Chemistry Pseudomonas aeruginosa Microorganism Pseudomonas Biosorption Pseudomonas nitroreducens Live biomass biology.organism_classification medicine.disease_cause Toxic Applied Microbiology and Biotechnology Bioaccumulation Pseudomonas alcaligenes Bioremediation Heavy metals medicine Dead biomass Food science TP248.13-248.65 Biotechnology |
Zdroj: | Biotechnology Reports, Vol 32, Iss, Pp e00685-(2021) |
Popis: | The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial isolates were obtained from wastewater bodies from an industrial zone in the municipality of Girardota, Antioquia, Colombia. Subsequently, they were selected to evaluate their lead tolerance capacity at different concentrations. In addition, some parameters were determined, such as the capacity to produce exopolysaccharides and their biosorption to understand potential mechanisms associated to lead tolerance. According to the biocehemical test (Vitek) and the molecular analysis of sequences of 16S rDNA, bacterial were identified as Pseudomonas aeruginosa, Pseudomonas nitroreducens, and Pseudomonas alcaligenes. We determined that the seven isolates had the capacity to tolerate concentrations higher than 50 mg/ml of lead, and that the concentration and exposure time (40 h) to this metal significantly affect the Pseudomonas spp. isolates. Statistically significant differences were detected (p |
Databáze: | OpenAIRE |
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