Neuroprotective Effects of Alpha-Lipoic Acid in Experimental Spinal Cord Injury in Rats
| Autor: | Hale Z. Toklu, Necat Biber, Can Erzik, Hasan Hüseyin Çelik, Şule Çetinel, Ayliz Velioğlu Öğünç, Tayfun Hakan, Dilek Akakin, Mehmet Erşahin, Göksel Şener, Esra Çikler |
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| Přispěvatelé: | Toklu, Hale Z., Hakan, Tayfun, Celik, Hasan, Biber, Necat, Erzik, Can, Ogunc, Ayliz V., Akakin, Dilek, Cikler, Esra, Cetinel, Sule, Ersahin, Mehmet, Sener, Goksel |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
METHYLPREDNISOLONE medicine.medical_treatment Original Contributions DIHYDROLIPOIC ACID ISCHEMIA-REPERFUSION INJURY medicine.disease_cause Antioxidants Lipid peroxidation PROTECTS chemistry.chemical_compound 0302 clinical medicine Malondialdehyde ANTIOXIDANT GLUTATHIONE Medicine 030212 general & internal medicine OXIDATIVE STRESS Spinal cord injury Neurologic Examination biology Thioctic Acid Neuroprotection medicine.anatomical_structure Neuroprotective Agents Anesthesia Myeloperoxidase medicine.medical_specialty Alpha-lipoic acid Intraperitoneal injection TRAUMATIC BRAIN-INJURY 030209 endocrinology & metabolism DNA Fragmentation Trauma 03 medical and health sciences Internal medicine Spinal cord injuries Animals Rats Wistar Peroxidase Analysis of Variance business.industry medicine.disease Spinal cord Rats Disease Models Animal Endocrinology chemistry Glutathione Myeloperoxidase Luminescent Measurements biology.protein DNA damage Neurology (clinical) Lipid Peroxidation business Reactive Oxygen Species Oxidative stress |
| Popis: | Background: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. Objective: To investigate the putative neuroprotective effect of a-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). Methods: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. Results: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SO-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. Conclusion: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation. |
| Databáze: | OpenAIRE |
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