Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein
| Autor: | Lauren E. Bortolotti, Luciana Renna, Federica Brandizzi, Giovanni Stefano, Vikram Misra, Sally L. Hanton |
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| Rok vydání: | 2005 |
| Předmět: |
Recombinant Fusion Proteins
Amino Acid Motifs Green Fluorescent Proteins Molecular Sequence Data Golgi Apparatus Plant Science Biology Transfection Plant Epidermis symbols.namesake Arabidopsis Chlorocebus aethiops Tobacco Genetics Animals Humans Amino Acid Sequence Golgi localization CASP Vero Cells Microscopy Confocal Sequence Homology Amino Acid Arabidopsis Proteins Golgi Matrix Proteins Membrane Proteins Lipid bilayer fusion General Medicine Golgi apparatus biology.organism_classification humanities Transmembrane protein Cell biology Protein Transport Transmembrane domain Biochemistry Golgi cisterna symbols Tyrosine Sequence Alignment Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 58:109-122 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-005-4618-4 |
| Popis: | Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane mammalian and yeast golgin. Similar to its homologues, the plant CASP contains a long N-terminal coiled-coil region protruding into the cytosol and a C-terminal transmembrane domain with amino acid residues which are highly conserved across species. Through fluorescent protein tagging experiments, we show that plant CASP localizes at the plant Golgi apparatus and that the C-terminus of this protein is sufficient for its localization, as has been shown for its mammalian counterpart. In addition, we demonstrate that the plant CASP is able to localize at the mammalian Golgi apparatus. However, mutagenesis of a conserved tyrosine in the transmembrane domain revealed that it is necessary for ER export and Golgi localization of the Arabidopsis CASP in mammalian cells, but is not required for its correct localization in plant cells. These data suggest that mammalian and plant cells have different mechanisms for concentrating CASP in the Golgi apparatus. |
| Databáze: | OpenAIRE |
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