Phosphorylation of DegU is essential for activation of amyE expression in Bacillus subtilis
| Autor: | Monica Gupta, K Krishnamurthy Rao |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Base Sequence
fungi Cell Amino Acid Motifs Molecular Sequence Data Swarming motility General Medicine Bacillus subtilis Gene Expression Regulation Bacterial Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology medicine.anatomical_structure Biochemistry Promoter activity Bacterial Proteins Genes Bacterial medicine Phosphorylation alpha-Amylases General Agricultural and Biological Sciences Promoter Regions Genetic Gene |
| Zdroj: | Journal of biosciences. 39(5) |
| ISSN: | 0973-7138 |
| Popis: | Alpha (α)-amylase (amyE) is one of the major exo-enzymes secreted by Bacillus subtilis during the post-exponential phase. The DegS-DegU two-component system regulates expression of majority of post-exponentially expressed genes in B. subtilis. It has been demonstrated that varying levels of the phosphorylated form of DegU (DegU-P) control different cellular processes. Exo-protease production is observed when effective concentration of DegU-P rises in the cell, whereas swarming motility is favoured at very low amounts of DegU-P. In this study we show that like other exo-proteases, expression of amyE is positively regulated by increase in DegU-P levels in the cell. We also demonstrate that residues at the DNA-binding helix-turn-helix (HTH) motif of DegU are necessary for the amyE expression. This observation is further reinforced by demonstrating the direct interaction of DegU on amyE promoter. |
| Databáze: | OpenAIRE |
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