Three-dimensional mouse brain cytoarchitecture revealed by laboratory-based x-ray phase-contrast tomography
Autor: | Mareike Töpperwien, Franziska Stöber, Anja M. Oelschlegel, Martin Krenkel, Daniel Vincenz, Jürgen Goldschmidt, Tim Salditt |
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Rok vydání: | 2017 |
Předmět: |
PHASE CONTRAST
0301 basic medicine Materials science 02 engineering and technology Matrix (biology) Article Mice 03 medical and health sciences Biological specimen Imaging Three-Dimensional Single-cell analysis PHASE CONTRAST IMAGING Animals HISTOLOGY Microscopy Phase-Contrast SOFT TISSUES Multidisciplinary PHASE CONTRAST MICROTOMOGRAPHY Resolution (electron density) X-ray Brain HISTOPATHOLOGY Anatomy 021001 nanoscience & nanotechnology Staining 030104 developmental biology FRELON CAMERA Cytoarchitecture Tomography Single-Cell Analysis Tomography X-Ray Computed 0210 nano-technology Algorithms Biomedical engineering |
Zdroj: | 'Scientific Reports ', vol: 7, pages: 42847-1-42847-8 (2017) Scientific reports, 7:42847 Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Studies of brain cytoarchitecture in mammals are routinely performed by serial sectioning of the specimen and staining of the sections. The procedure is labor-intensive and the 3D architecture can only be determined after aligning individual 2D sections, leading to a reconstructed volume with non-isotropic resolution. Propagation-based x-ray phase-contrast tomography offers a unique potential for high-resolution 3D imaging of intact biological specimen due to the high penetration depth and potential resolution. We here show that even compact laboratory CT at an optimized liquid-metal jet microfocus source combined with suitable phase-retrieval algorithms and a novel tissue preparation can provide cellular and subcellular resolution in millimeter sized samples of mouse brain. We removed water and lipids from entire mouse brains and measured the remaining dry tissue matrix in air, lowering absorption but increasing phase contrast. We present single-cell resolution images of mouse brain cytoarchitecture and show that axons can be revealed in myelinated fiber bundles. In contrast to optical 3D techniques our approach does neither require staining of cells nor tissue clearing, procedures that are increasingly difficult to apply with increasing sample and brain sizes. The approach thus opens a novel route for high-resolution high-throughput studies of brain architecture in mammals. |
Databáze: | OpenAIRE |
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