Platelet binding and phagocytosis by macrophages
| Autor: | Bahram A Badlou, Jan-Willem N. Akkerman, Ya Ping Wu, W Martin Smid |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cell Survival
Phagocytosis Immunology Platelet Transfusion Biology Cell Line chemistry.chemical_compound Platelet Adhesiveness Platelet adhesiveness Macrophages/metabolism Immunology and Allergy Macrophage Humans Platelet Macrophages Hematology Phosphatidylserine Cell sorting Cell biology P-Selectin P-Selectin/metabolism chemistry Biochemistry Cell culture Blood Preservation Blood Preservation/adverse effects Phorbol |
| Zdroj: | Transfusion, 46(8), 1432-43. Wiley |
| ISSN: | 0041-1132 |
| DOI: | 10.1111/j.1537-2995.2006.00913.x |
| Popis: | BACKGROUND: Earlier it was reported that metabolic arrest followed by incubation at 4 degrees C reduces the platelet (PLT) storage defect. Here it is reported that this treatment also reduces binding and phagocytosis by macrophages.STUDY DESIGN AND METHODS: Phagocytosis of mepacrine-labeled PLTs by macrophages changes the latter into bright fluorescent particles easily detected by fluorescence-activated cell sorting.RESULTS: In combination with conventional binding analysis it was found that binding to phorbol 12-myristate 13-acetate-matured THP-1 cells is primarily regulated by PLT P-selectin expression and phagocytosis by combined phosphatidylserine (PS) exposure and glycoprotein (GP) Ibalpha clustering. It was found that trapping of PLT Ca2+ and raising cAMP reduces phagocytosis by lowering PS exposure. Chilling of PLTs leads to an increase in binding and PS- and GPIbalpha-mediated phagocytosis. Prior depletion of PLT energy stores prevents this increase by preserving low Ca2+ concentration, PS exposure, and PS-mediated phagocytosis.CONCLUSION: These data characterize the individual factors that control PLT binding and phagocytosis and might help to define conditions that improve the survival of stored PLTs after transfusion. |
| Databáze: | OpenAIRE |
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