Sequential expression of CD34 and CD33 antigens on myeloid colony-forming cells
| Autor: | Kalizia Katrilaka, Hans-Jörg Bühring, Busch Fw, Gabriele Hummel, Bernhard Asenbauer |
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| Rok vydání: | 2009 |
| Předmět: |
Myeloid
Cellular differentiation Sialic Acid Binding Ig-like Lectin 3 CFU-GM Antigens Differentiation Myelomonocytic Antigens CD34 Hematology General Medicine Cell sorting Biology Hematopoietic Stem Cells Antigens Differentiation Molecular biology Colony-Forming Units Assay medicine.anatomical_structure Antigen Antigens CD Bone Marrow Immunology medicine Humans Bone marrow Myelopoiesis Progenitor cell |
| Zdroj: | European Journal of Haematology. 42:143-149 |
| ISSN: | 1600-0609 0902-4441 |
| DOI: | 10.1111/j.1600-0609.1989.tb01203.x |
| Popis: | The expression of CD33 and CD34 antigens on human bone marrow cells was examined by fluorescence-activated cell sorting and colony assays. A marked difference of antigen expression was observed on sorted progenitors of the granulocyte/macrophage lineage (CFU-GM) with respect to enrichment and maturation status. Single-color cell sorting revealed no difference of enrichment by anti-CD33 antibody between day-7 and d-14 progenitors, while anti-CD34 antibody preferentially enriched d-14 colony-forming units (CFU). By two-color cell sorting it could be shown that enrichment of d-14 CFU-GM occurred mostly in the CD34+CD33- and to a lesser extent in the double-positive fraction. In contrast, there was no difference in degree of enrichment between d-7 and d-14 CD34-CD33+ CFU-GM. From these data we conclude that, during early myelopoiesis, CD34 antigens are gradually lost while CD33 antigens are acquired. |
| Databáze: | OpenAIRE |
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