New class of IMP cyclohydrolases in Methanococcus jannaschii
| Autor: | Robert H. White, Marion Graupner, Huimin Xu |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Methanococcus
Ribonucleotide Physiology and Metabolism Molecular Sequence Data medicine.disease_cause Microbiology Genes Archaeal medicine Molecular Biology Gene Escherichia coli chemistry.chemical_classification Genetics biology IMP cyclohydrolase Sequence Analysis DNA biology.organism_classification enzymes and coenzymes (carbohydrates) Enzyme DNA Archaeal chemistry Nucleotide Deaminases IMP cyclohydrolase activity bacteria Archaea |
| Zdroj: | Journal of bacteriology. 184(5) |
| ISSN: | 0021-9193 |
| Popis: | The enzyme responsible for observed IMP cyclohydrolase activity in Methanococcus jannaschii was purified and sequenced: its genetic locus was found to correspond to gene MJ0626. The MJ0626 gene was cloned, and its protein product was expressed in Escherichia coli and shown to catalyze the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP. The enzyme has no sequence similarity to known enzymes, and its catalytic properties appear distinct from any characterized IMP cyclohydrolase. The purO gene for the enzyme is currently found only in the domain Archaea . |
| Databáze: | OpenAIRE |
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