An antibody panel for immunohistochemical analysis of the retina in Davidson's-fixed, paraffin-embedded eyes of rats
| Autor: | Elizabeth Johnson, Kerry Ratcliffe, Jennifer S. McKay, Gulshanara Ahmed, Samantha J. Steele |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Rhodopsin Synaptophysin Apoptosis Toxicology Eye Sensitivity and Specificity Pathology and Forensic Medicine Histones chemistry.chemical_compound Glutamate-Ammonia Ligase Glial Fibrillary Acidic Protein medicine Animals Rats Long-Evans Fixative Fixation (histology) Cell Proliferation Retina Paraffin Embedding biology Glial fibrillary acidic protein Staining and Labeling Caspase 3 Antibodies Monoclonal Retinal Cell Biology General Medicine Immunohistochemistry Staining Rats medicine.anatomical_structure chemistry biology.protein sense organs Biomarkers |
| Zdroj: | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 61(2) |
| ISSN: | 1618-1433 |
| Popis: | Unlike most other tissues, the optimal fixative for preserving eye morphology is considered to be Davidson's fixative or modified Davidson's rather than formalin. However, the methodology for antibodies to be used in tissues fixed this way is not normally outlined in current antibody datasheets. Additionally, where eyes have been stored in Davidson's fixative, the efficacy of retrospective analysis of eye morphology by immunohistochemistry is largely unknown. The aim of this study was to compare a panel of six antibodies in both Davidson's-fixed and formalin-fixed pigmented and non-pigmented rat eyes, in order to provide optimal methods for future retinal immunohistochemical evaluation with image analysis. The antibodies evaluated were raised against rhodopsin, synaptophysin, glutamine synthetase, glial fibrillary acidic protein (GFAP), cleaved caspase-3 and phospho-histone H3 (PH3). Overall, the staining quality of these antibodies was found to be optimal in Davidson's compared to formalin-fixed tissues after a time period of up to 4 days in fixative. The methods outlined thus provide a platform for future detailed analysis of retinal pathology in Davidson's-fixed eyes. |
| Databáze: | OpenAIRE |
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