Osteogenic potential of adipogenic predifferentiated human bone marrow‐derived multipotent stromal cells for bone tissue‐engineering

Autor: Hanane El-Hafci, Hervé Petite, Adrien Moya, Nathanaël Larochette, Delphine Logeart-Avramoglou, Esther Potier, Marianne Bourguignon
Přispěvatelé: Bioingénierie et Bioimagerie Ostéo-articulaires, Biomécanique et Biomatériaux Ostéo-Articulaires (B2OA (UMR_7052)), École nationale vétérinaire d'Alfort (ENVA)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Stromal cell
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Cell Survival
Cell
Population
Biomedical Engineering
Mice
Nude
Neovascularization
Physiologic
Medicine (miscellaneous)
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
bone
Bone and Bones
predifferentiation
adipogenesis
osteogenesis
Biomaterials
MSC
03 medical and health sciences
Paracrine signalling
Tissue engineering
Ischemia
In vivo
Cell Adhesion
medicine
Animals
Humans
Cell Lineage
education
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
education.field_of_study
Osteoblasts
Chemotactic Factors
Tissue Engineering
Chemistry
Mesenchymal Stem Cells
Coculture Techniques
In vitro
tissue-engineering
Cell biology
030104 developmental biology
medicine.anatomical_structure
Adipogenesis
Immunology
Female
Biomarkers
Zdroj: Journal of Tissue Engineering and Regenerative Medicine
Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2018, 12 (3), ⟨10.1002/term.2571⟩
ISSN: 1932-7005
1932-6254
DOI: 10.1002/term.2571⟩
Popis: International audience; In the present study, we evaluated the benefits of an adipogenic predifferentiation, the pathway most closely related to osteoblastogenesis, on the pro-osteogenic potential of human adult multipotent bone marrow stromal cells (hBMSCs), both in vitro and in vivo. Adipogenic differentiation of hBMSCs for 14 days resulted in a heterogeneous cell population from which the most adipogenic-committed cells were eliminated by their lack of readhesion ability. Our results provided evidence that the select adherent adipogenic differentiated hBMSCs (sAD+ cells) express a gene profile characteristic of both adipogenic and osteogenic lineages. In vitro, when cultured in osteogenic medium, sAD+ differentiated along the osteogenic lineage faster than undifferentiated hBMSCs. In vivo, in an ectopic mouse model, sAD+ exhibited a significantly higher bone formation capability compared with undifferentiated hBMSCs. We sought, then, to investigate the underlying mechanisms responsible for such beneficial effects of adipogenic predifferentiation on bone formation and found that this outcome was not linked to a better cell survival post-implantation. The secretome of sAD+ was both proangiogenic and chemoattractant, but its potential did not supersede the one of undifferentiated hBMSCs. However, using co-culture systems, we observed that the sAD+ paracrine factors were pro-osteogenic on undifferentiated hBMSCs. In conclusion, adipogenic priming endows hBMSCs with high osteogenic potential as well as pro-osteogenic paracrine-mediated activity. This preconditioning appears as a promising strategy for bone tissue engineering technology in order to improve the hBMSC osteogenic potency in vivo.
Databáze: OpenAIRE
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