Carbon-13 and proton nuclear magnetic resonance of unsonicated model and mitochondrial membranes
| Autor: | D. Chapman, P. Beynon, Eric Oldfield, K. M. Keough |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Chloroplasts
Magnetic Resonance Spectroscopy food.ingredient Eggs Molecular Conformation Mitochondria Liver Biochemistry Lecithin chemistry.chemical_compound food Nuclear magnetic resonance Animals Molecular Biology Brain Chemistry Carbon Isotopes Egg lecithin Membranes Chemistry Organic Chemistry Carbon-13 technology industry and agriculture Membrane structure Membranes Artificial Cell Biology Plants Rats Chloroplast Cholesterol Membrane Phosphatidylcholines Proton NMR Cattle lipids (amino acids peptides and proteins) Sphingomyelin Chickens |
| Zdroj: | Chemistry and Physics of Lipids. 10:37-50 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/0009-3084(73)90039-x |
| Popis: | Proton nuclear magnetic resonance (PMR) spectra at 270 MHz of aqueous dispersions of nonsonicated egg lecithin, dipalmitoyl lecithin, egg lecithin-cholesterol (1 : 1) and dipalmitoyl lecithin-cholesterol (1 : 1), together with PMR spectra of mitochondrial membranes and their extracted lipids, have been obtained. Carbon-13 nuclear magnetic resonance (CMR) spectra at 25.2 MHz of egg lecithin, egg lecithin-cholesterol (1 : 1) and sphingomyelin, together with CMR spectra of chloroplast and mitochondrial membranes, and erythrocyte ghosts, have also been obtained. The results obtained using CMR appear very promising for further study of intact membrane structure. It is suggested, on the basis of CMR evidence, that the proteins in mitochondrial membranes may be considerably less mobile than the lipids . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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