A human genotyping trial to estimate the post-feeding time from mosquito blood meals

Autor: Toshimichi Yamamoto, Masaaki Hara, Nakayama Koji, Hikitsuchi Tomoyuki, Kawajiri Yumi, Aya Takada, Mitsuo Umeda, Atsushi Nagai, Yuuji Hiroshige, Akira Ishii, Koichi Suzuki
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Veterinary medicine
Genotyping Techniques
Physiology
lcsh:Medicine
Artificial Gene Amplification and Extension
Disease Vectors
Mosquitoes
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
Aedes
Abdomen
Medicine and Health Sciences
lcsh:Science
DNA extraction
Polymerase chain reaction
Genetics
Multidisciplinary
biology
Genomics
Body Fluids
Insects
Culex
Tandem Repeats
Blood
Infectious Diseases
Microsatellite
Animal Nutritional Physiological Phenomena
Female
Anatomy
Research Article
Genotyping
Aedes albopictus
Arthropoda
030231 tropical medicine
Research and Analysis Methods
03 medical and health sciences
Extraction techniques
Animals
Humans
Repeated Sequences
Molecular Biology Techniques
Molecular Biology
lcsh:R
Organisms
Biology and Life Sciences
DNA
biology.organism_classification
Blood meal
Invertebrates
Insect Vectors
Species Interactions
030104 developmental biology
Genetic Loci
lcsh:Q
Reagent Kits
Diagnostic
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 6, p e0179319 (2017)
ISSN: 1932-6203
Popis: Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus. After stereomicroscopic observation, the extracted DNA samples were quantified using a human DNA quantification and quality control kit and were genotyped for 15 short tandem repeats using a commercial multiplexing kit. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days. Furthermore, the quantitative ratios derived from STR peak heights and the quality control kit (Q129/Q41, Q305/Q41, and Q305/Q129) were reasonably effective for estimating the approximate post-feeding time after 2-3 days. We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately.
Databáze: OpenAIRE
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