Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers
| Autor: | Quênia Gramile Silva Meira, Maria Lúcia da Conceição, José Pinto de Siqueira Júnior, Isabella de Medeiros Barbosa, Evandro Leite de Souza, Ana Júlia Alves Aguiar Athayde |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Staphylococcus aureus
Time Factors Food Handling Sodium Hypochlorite lcsh:QR1-502 Microbial Sensitivity Tests medicine.disease_cause food processing environment Microbiology lcsh:Microbiology biofilm Bacterial Adhesion Incubation period chemistry.chemical_compound Peracetic acid medicine Environmental Microbiology Food science Peracetic Acid Polypropylene Biofilm Temperature Biofilm matrix Adhesion cross-contamination chemistry Sodium hypochlorite Biofilms sanitizers Research Paper Disinfectants |
| Zdroj: | Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.45 n.1 2014 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 45, Iss 1, Pp 67-75 (2014) Brazilian Journal of Microbiology, Volume: 45, Issue: 1, Pages: 67-75, Published: 2014 |
| ISSN: | 1678-4405 1517-8382 |
| Popis: | This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7 ºC. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered on polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacity to adhere and form biofilm on polypropylene and stainless steel surfaces under different growth conditions. Moreover, the cells in biofilm matrix were resistant for total removal when submitted to the exposure to sanitizers. |
| Databáze: | OpenAIRE |
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