Secreted aspartic peptidases of Candida albicans liberate bactericidal hemocidins from human hemoglobin
| Autor: | Oliwia Bochenska, Maria Rąpała-Kozik, Natalia Wolak, Grażyna Braś, Mitsuyoshi Ueda, Adam Dubin, Andrzej Kozik, Paweł Mak, Wataru Aoki |
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| Rok vydání: | 2013 |
| Předmět: |
secreted aspartic peptidases
Physiology Proteolysis Virulence Biology medicine.disease_cause Biochemistry Microbiology Cellular and Molecular Neuroscience Hemoglobins Endocrinology Lactobacillus acidophilus bactericidal Candida albicans medicine Escherichia coli Aspartic Acid Endopeptidases Humans Menstrual Cycle chemistry.chemical_classification medicine.diagnostic_test hemoglobin Hydrogen-Ion Concentration Antimicrobial biology.organism_classification Corpus albicans Peptide Fragments Amino acid antibacterial peptidases hemocidins vagina chemistry Vagina peptides Female menstruation Peptides Sap microbicidal |
| Zdroj: | Peptides. 48 |
| ISSN: | 1873-5169 |
| Popis: | Secreted aspartic peptidases (Saps) are a group of ten acidic hydrolases considered as key virulence factors of Candida albicans . These enzymes supply the fungus with nutrient amino acids as well as are able to degrade the selected host's proteins involved in the immune defense. Our previous studies showed that the human menstrual discharge is exceptionally rich in bactericidal hemoglobin (Hb) fragments – hemocidins. However, to date, the genesis of such peptides is unclear. The presented study demonstrates that the action of C. albicans isozymes Sap1–Sap6, Sap8 and Sap9, but not Sap7 and Sap10, toward human hemoglobin leads to limited proteolysis of this protein and generates a variety of antimicrobial hemocidins. We have identified these peptides and checked their activity against selected microorganisms representative for human vagina. We have also demonstrated that the process of Hb hydrolysis is most effective at pH 4.0, characteristic for vagina, and the liberated peptides showed pronounced killing activity toward Lactobacillus acidophilus , and to a lower degree, Escherichia coli . However, only a very weak activity toward Staphylococcus aureus and C. albicans was noticed. These findings provide interesting new insights into pathophysiology of human vaginal candidiasis and suggest that C. albicans may be able to compete with the other microorganisms of the same physiological niche using the microbicidal peptides generated from the host protein. |
| Databáze: | OpenAIRE |
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