A pilot study to examine the association between human gut microbiota and the host's central obesity
| Autor: | Magdalin Cheong, Collins Wenhan Chu, Seok Hwee Koo, Edmund J.D. Lee, Ngai Moh Law, Joan Joo Ching Khoo, Gaik Hong Soon, Kwong Ming Fock, John Chen Hsiang, Tiing Leong Ang, Eliza Xin Pei Ho, Paola Florez de Sessions |
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| Rok vydání: | 2019 |
| Předmět: |
obesity
Rikenellaceae Saturated fat Physiology RC799-869 metabolic syndrome 03 medical and health sciences 0302 clinical medicine Insulin resistance Diabetes mellitus microbiota Medicine Microbiome Hepatology biology Adiponectin business.industry Gastroenterology Original Articles Diseases of the digestive system. Gastroenterology continental population groups biology.organism_classification medicine.disease Obesity 030220 oncology & carcinogenesis Original Article 030211 gastroenterology & hepatology Metabolic syndrome diet business |
| Zdroj: | JGH Open, Vol 3, Iss 6, Pp 480-487 (2019) JGH Open: An Open Access Journal of Gastroenterology and Hepatology |
| ISSN: | 2397-9070 |
| DOI: | 10.1002/jgh3.12184 |
| Popis: | Background and Aim Perturbance in the composition of human gut microbiota has been associated with metabolic disorders such as obesity, diabetes mellitus, and insulin resistance. The objectives of this study are to examine the effects of ethnicity, central obesity, and recorded dietary components on potentially influencing the human gut microbiome. We hypothesize that these factors have an influence on the composition of the gut microbiome. Methods Subjects of Chinese (n = 14), Malay (n = 10), and Indian (n = 11) ancestry, with a median age of 39 years (range: 22–70 years old), provided stool samples for gut microbiome profiling using 16S rRNA sequencing and completed a dietary questionnaire. The serum samples were assayed for a panel of biomarkers (interleukin‐6, tumor necrosis factor alpha, adiponectin, cleaved cytokeratin 18, lipopolysaccharide‐binding protein, and limulus amebocyte lysate). Central obesity was defined by waist circumference cut‐off values for Asians. Results There were no significant differences in Shannon alpha diversity for ethnicity and central obesity and no associations between levels of inflammatory cytokines and obesity. The relative abundances of Anaerofilum (P = 0.02), Gemellaceae (P = 0.02), Streptococcaceae (P = 0.03), and Rikenellaceae (P = 0.04) were significantly lower in the obese group. From principle coordinate analysis, the effects of the intake of fiber and fat/saturated fat were in contrast with each other, with clustering of obese individuals leaning toward fiber. Conclusion The study demonstrated that there were differences in the gut microbiome in obese individuals. Certain bacterial taxa were present in lower abundance in the group with central obesity. Fiber and fat/saturated fat diets were not the key determinants of central obesity. The objectives of this study are to examine the effects of ethnicity, central obesity, and dietary components on the human gut microbiome. Asian subjects (n = 35) provided stool samples for gut microbiome profiling using 16S rRNA sequencing and completed a dietary questionnaire. The relative abundances of Anaerofilum (P = 0.02), Gemellaceae (P = 0.02), Streptococcaceae (P = 0.03), and Rikenellaceae (P = 0.04) were significantly lower in the obese group. |
| Databáze: | OpenAIRE |
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