Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe
| Autor: | Dilyana B. Mangarova, Bernd Hamm, Lisa C. Adams, Rebecca Buchholz, Carolin Reimann, Julia Brangsch, Heike Traub, Marcus R. Makowski, Jing Zhao, Jana Moeckel, Jessica Saatz, Avan Kader, Jan O. Kaufmann, Uwe Karst |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty QH301-705.5 magnetic resonance imaging molecular imaging prostate cancer 570 Biologie Biology General Biochemistry Genetics and Molecular Biology Article Prostate cancer In vivo Prostate ddc:570 medicine Biology (General) General Immunology and Microbiology medicine.diagnostic_test Cancer Magnetic resonance imaging medicine.disease medicine.anatomical_structure biology.protein Molecular imaging General Agricultural and Biological Sciences Molecular probe Elastin |
| Zdroj: | Biology, Vol 10, Iss 1217, p 1217 (2021) Biology Biology; Volume 10; Issue 11; Pages: 1217 |
| ISSN: | 2079-7737 |
| Popis: | Simple Summary One of the most commonly diagnosed cancers in men is prostate cancer (PCa). Understanding tumor progression can help diagnose and treat the disease at an early stage. Components of the extracellular matrix (ECM) play a key role in the development and progression of PCa. Elastin is an essential component of the ECM and constantly changes during tumor development. This article visualizes and quantifies elastin in magnetic resonance imaging (MRI) using a small molecule probe. Results were correlated with histological examinations. Using an elastin-specific molecular probe, we were able to make predictions about the cellular structure in relation to elastin and thus draw conclusions about the size of the tumor, with smaller tumors having a higher elastin content than larger tumors. Abstract Human prostate cancer (PCa) is a type of malignancy and one of the most frequently diagnosed cancers in men. Elastin is an important component of the extracellular matrix and is involved in the structure and organization of prostate tissue. The present study examined prostate cancer in a xenograft mouse model using an elastin-specific molecular probe for magnetic resonance molecular imaging. Two different tumor sizes (500 mm3 and 1000 mm3) were compared and analyzed by MRI in vivo and histologically and analytically ex vivo. The T1-weighted sequence was used in a clinical 3-T scanner to calculate the relative contrast enhancement before and after probe administration. Our results show that the use of an elastin-specific probe enables better discrimination between tumors and surrounding healthy tissue. Furthermore, specific binding of the probe to elastin fibers was confirmed by histological examination and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS). Smaller tumors showed significantly higher signal intensity (p > 0.001), which correlates with the higher proportion of elastin fibers in the histological evaluation than in larger tumors. A strong correlation was seen between relative enhancement (RE) and Elastica–van Gieson staining (R2 = 0.88). RE was related to inductively coupled plasma–mass spectrometry data for Gd and showed a correlation (R2 = 0.78). Thus, molecular MRI could become a novel quantitative tool for the early evaluation and detection of PCa. |
| Databáze: | OpenAIRE |
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