Characterization of polyamine homeostasis in l-ornithine-induced acute pancreatitis in rats
| Autor: | Sándor Dósa, Andrea Siska, Péter Hegyi, Riitta Sinervirta, Viktória Venglovecz, Béla Iványi, Sándor Berczi, Zoltán Rakonczay, Tamás Takács, György Biczó, Leena Alhonen |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Ornithine medicine.medical_specialty Endocrinology Diabetes and Metabolism Interleukin-1beta Spermine HSP72 Heat-Shock Proteins Ornithine decarboxylase chemistry.chemical_compound Endocrinology NF-KappaB Inhibitor alpha Acetyltransferases Internal medicine Internal Medicine medicine Animals Homeostasis Rats Wistar Lung Pancreas Hepatology business.industry Biogenic Polyamines medicine.disease Rats Spermidine Polyamine Catabolism Polyamine Analogue chemistry Liver Pancreatitis Creatinine Acute Disease Polyamine homeostasis I-kappa B Proteins Polyamine business |
| Zdroj: | Pancreas. 39(7) |
| ISSN: | 1536-4828 |
| Popis: | OBJECTIVES l-Ornithine is a precursor of polyamine synthesis that is essential for cell survival. In contrast, intraperitoneal (IP) administration of a large dose of l-ornithine results in death of pancreatic acinar cells in rats. We investigated changes in pancreatic and extrapancreatic polyamine homeostasis after injection of l-ornithine and tested the effects of the stable polyamine analogue methylspermidine (MeSpd) on l-ornithine-induced pancreatitis. METHODS Male Wistar rats were injected IP with 3 g/kg l-ornithine and were untreated, pretreated, or treated with 50 mg/kg MeSpd IP. Rats were killed after 0 to 168 hours for determinations of polyamines and activities of ornithine decarboxylase and spermidine/spermine N(1)-acetyltransferase (SSAT). Pancreatitis severity was assessed by measuring standard laboratory and histological parameters. RESULTS Injection of l-ornithine paradoxically induced pancreatic spermidine catabolism, possibly via activation of SSAT, after (>6 hours) appearance of the first histological signs of acute pancreatitis. Polyamine levels generally increased in the lung and liver with the exception of lung spermidine levels, which decreased. Methylspermidine did not influence polyamine levels and SSAT activity and did not ameliorate the severity of l-ornithine-induced pancreatitis. CONCLUSIONS l-Ornithine-induced pancreatitis was associated with activation of pancreatic polyamine catabolism. However, administration of a metabolically stable polyamine analogue did not affect disease severity. |
| Databáze: | OpenAIRE |
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