Proteomic markers of low and high fertility bovine spermatozoa separated by Percoll gradient
| Autor: | Ezequiel Calvo, Patrick Blondin, Robert Sullivan, Sylvie Bourassa, Patrick Vincent, Olivier D'Amours |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_treatment Population Context (language use) Semen Cell Separation Centrifugation Isopycnic Biology Insemination GPX5 Andrology 03 medical and health sciences 0302 clinical medicine Genetics medicine Animals education reproductive and urinary physiology education.field_of_study 030219 obstetrics & reproductive medicine urogenital system Artificial insemination Povidone Cell Biology Silicon Dioxide Antigens Differentiation Spermatozoa Sperm Fertility 030104 developmental biology Cattle Percoll Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 86:999-1012 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.23174 |
| Popis: | In the context of artificial insemination, male fertility is defined as the ability to produce functional spermatozoa able to withstand cryopreservation. We hypothesized that interindividual variations in fertility depend on the proportion of the fully functional sperm population contained in the insemination dose. The objective of this study was to identify protein markers of the fully functional sperm subpopulation. Insemination doses from four high-fertility (HF) and four low-fertility (LF) bulls with comparable post-thaw quality parameters were selected for proteomic analysis using iTRAQ technology. Thawed semen was centrifuged through a Percoll gradient to segregate the motile (high density [HD]) from the immotile (low density [LD]) sperm populations. Sperm proteins were extracted with sodium deoxycholate and four groups were compared: LD and HD spermatozoa from LF and HF bulls. A total of 498 unique proteins were identified and quantified. Comparison of HD spermatozoa from HF and LF bulls revealed that five proteins were significantly more abundant in the HF group (AK8, TPI1, TSPAN8, OAT, and DBIL5) whereas five proteins were more abundant in the LF group (RGS22, ATP5J, CLU, LOC616319, and CCT5). Comparison of LD spermatozoa from HF and LF bulls revealed that four proteins were significantly more abundant in the HF group (IL4I1, CYLC2, OAT, and ARMC3) whereas 15 proteins were significantly more abundant in the LF group (HADHA, HSP90AA1, DNASE1L3, SLC25A20, GPX5, TCP1, HIP1, CLU, G5E622, LOC616319, HSPA2, NUP155, DPY19L2, SPERT, and SERPINE2). DBIL5, TSPAN8, and TPI1 showed potential as putative markers of the fully functional sperm subpopulation. |
| Databáze: | OpenAIRE |
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