Bacterial isolation and antibiotic susceptibility from diabetic foot ulcers in Kenya using microbiological tests and comparison with RT-PCR in detection of S. aureus and MRSA
Autor: | Daniel M. Mutonga, Marianne W. Mureithi, Nancy Ngugi, Fredrick C Otieno |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Multi-drug resistant organisms Meticillin Ceftazidime lcsh:Medicine 0302 clinical medicine Ampicillin Drug Resistance Multiple Bacterial Medicine 030212 general & internal medicine lcsh:QH301-705.5 Clindamycin General Medicine Bacterial Infections Diabetic Foot Anti-Bacterial Agents Bacterial Typing Techniques Polymerase chain reaction Research Note Klebsiella pneumoniae Pseudomonas aeruginosa Diabetic foot ulcers Macrolides medicine.drug Methicillin-Resistant Staphylococcus aureus Staphylococcus aureus Cefepime Microbial Sensitivity Tests Penicillins Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences Methicillin-resistant S. aureus Sulfanilamides Escherichia coli Humans lcsh:Science (General) Proteus mirabilis business.industry lcsh:R Amoxicillin Kenya Cephalosporins 030104 developmental biology Cross-Sectional Studies lcsh:Biology (General) business Cefuroxime Piperacillin lcsh:Q1-390 |
Zdroj: | BMC Research Notes, Vol 12, Iss 1, Pp 1-6 (2019) BMC Research Notes |
ISSN: | 1756-0500 |
Popis: | Objectives Diabetic foot ulcers (DFUs) often lead to hospital admissions, amputations and deaths; however, there is no up-to-date information on microbial isolates from DFUs and no mention of utilization of molecular techniques in Sub-Saharan Africa. We conducted a cross-sectional study among 83 adult patients at a tertiary hospital in Kenya over 12 months. The study aimed to isolate, identify bacteria, their antibiotic susceptibility patterns in active DFUs, and to compare standard microbiological methods versus a real-time PCR commercial kit in the detection of Staphylococcus aureus DNA and methicillin-resistant S. aureus (MRSA) DNA. Results Eighty swabs (94%) were culture-positive; 29% were Gram-positive and 65% were Gram-negative. The main organisms isolated were S. aureus (16%), Escherichia coli (15%), Proteus mirabilis (11%), Klebsiella pneumoniae (7%) and Pseudomonas aeruginosa (7%). The bacterial isolates showed resistance to commonly used antibiotics such as ampicillin, amoxicillin, cefepime, ceftazidime, cefuroxime, clindamycin, erythromycin, piperacillin–tazobactam, tetracycline and trimethoprim–sulphamethoxazole (TMPSMX). Thirty-one percent of the S. aureus isolated and 40% of the Gram-negatives were multi-drug resistant organisms (MDROs). There was a high prevalence of nosocomial bacteria. MRSA were not identified using culture methods but were identified using PCR. PCR was more sensitive but less specific than culture-based methods to identify S. aureus. Electronic supplementary material The online version of this article (10.1186/s13104-019-4278-0) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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