Bacterial isolation and antibiotic susceptibility from diabetic foot ulcers in Kenya using microbiological tests and comparison with RT-PCR in detection of S. aureus and MRSA

Autor: Daniel M. Mutonga, Marianne W. Mureithi, Nancy Ngugi, Fredrick C Otieno
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Multi-drug resistant organisms
Meticillin
Ceftazidime
lcsh:Medicine
0302 clinical medicine
Ampicillin
Drug Resistance
Multiple
Bacterial
Medicine
030212 general & internal medicine
lcsh:QH301-705.5
Clindamycin
General Medicine
Bacterial Infections
Diabetic Foot
Anti-Bacterial Agents
Bacterial Typing Techniques
Polymerase chain reaction
Research Note
Klebsiella pneumoniae
Pseudomonas aeruginosa
Diabetic foot ulcers
Macrolides
medicine.drug
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Cefepime
Microbial Sensitivity Tests
Penicillins
Real-Time Polymerase Chain Reaction
General Biochemistry
Genetics and Molecular Biology
Microbiology
03 medical and health sciences
Methicillin-resistant S. aureus
Sulfanilamides
Escherichia coli
Humans
lcsh:Science (General)
Proteus mirabilis
business.industry
lcsh:R
Amoxicillin
Kenya
Cephalosporins
030104 developmental biology
Cross-Sectional Studies
lcsh:Biology (General)
business
Cefuroxime
Piperacillin
lcsh:Q1-390
Zdroj: BMC Research Notes, Vol 12, Iss 1, Pp 1-6 (2019)
BMC Research Notes
ISSN: 1756-0500
Popis: Objectives Diabetic foot ulcers (DFUs) often lead to hospital admissions, amputations and deaths; however, there is no up-to-date information on microbial isolates from DFUs and no mention of utilization of molecular techniques in Sub-Saharan Africa. We conducted a cross-sectional study among 83 adult patients at a tertiary hospital in Kenya over 12 months. The study aimed to isolate, identify bacteria, their antibiotic susceptibility patterns in active DFUs, and to compare standard microbiological methods versus a real-time PCR commercial kit in the detection of Staphylococcus aureus DNA and methicillin-resistant S. aureus (MRSA) DNA. Results Eighty swabs (94%) were culture-positive; 29% were Gram-positive and 65% were Gram-negative. The main organisms isolated were S. aureus (16%), Escherichia coli (15%), Proteus mirabilis (11%), Klebsiella pneumoniae (7%) and Pseudomonas aeruginosa (7%). The bacterial isolates showed resistance to commonly used antibiotics such as ampicillin, amoxicillin, cefepime, ceftazidime, cefuroxime, clindamycin, erythromycin, piperacillin–tazobactam, tetracycline and trimethoprim–sulphamethoxazole (TMPSMX). Thirty-one percent of the S. aureus isolated and 40% of the Gram-negatives were multi-drug resistant organisms (MDROs). There was a high prevalence of nosocomial bacteria. MRSA were not identified using culture methods but were identified using PCR. PCR was more sensitive but less specific than culture-based methods to identify S. aureus. Electronic supplementary material The online version of this article (10.1186/s13104-019-4278-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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