Recent advances in radioimmunoassay technology for the juvenile hormones
| Autor: | Richard Ebersohl, Walter G. Goodman, Noelle A. Granger, Kiyoshi Hiruma, Anthony P. Orth, Yock C. Toong |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Physiology Radioimmunoassay Moths Biochemistry Antibodies Antibody Specificity Internal medicine Hemolymph medicine Animals Transferase Antiserum biology General Medicine biology.organism_classification Juvenile Hormones Titer Endocrinology Insect Science Hyalophora cecropia Juvenile hormone Rabbits Hormone |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 30:295-306 |
| ISSN: | 1520-6327 0739-4462 |
| DOI: | 10.1002/arch.940300215 |
| Popis: | Recent refinements in juvenile hormone radioimmunoassay technology now make this method significantly more sensitive and easier to use. Rabbit poly-clonal antisera against (10R) JH III and racemic JH II have been developed to determine hemolymph hormone titers in the low picogram range. The antisera display minimal cross-reactivity with JH metabolites, JH analogs, and hemolymph lipids. One antiserum recognizes racemic JH I, II, and (10R) III almost equivalently, exhibiting 50% displacement between 100 and 130 pg per tube. Another antiserum is JH II-specific and exhibits 50% displacement at 35 pg per tube. Assay sensitivity has been enhanced by using (10R,11S) [methyl−3H]-JH II of very high specific activity (> 80 Ci/mmol) generated with Hyalophora cecropia accessory gland S-adenosylmethionine transferase and S-[methyl−3H]-adenosyl-L-methionine. Preparation of biological samples has been simplified with overall recoveries of JH from hemolymph ranging between 60 and 75%. © 1995 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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