Role of serum factors and adherent cells in cloning of human T lymphocytes in agar culture
| Autor: | Hans-Dieter Flad, H.G. Opitz, A.J. Ulmer, Mogens H. Claesson |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
food.ingredient
T cell T-Lymphocytes chemistry.chemical_compound food Culture Techniques Conditioned medium medicine Cell Adhesion Agar Humans Bovine serum albumin 2-Mercaptoethanol Mercaptoethanol Cloning biology Serum Albumin Bovine Hematology General Medicine Molecular biology Clone Cells medicine.anatomical_structure chemistry Colony formation Mitogen-activated protein kinase biology.protein Cell Division |
| Zdroj: | Acta haematologica. 62(5-6) |
| ISSN: | 0001-5792 |
| Popis: | In the presence of serum, 2-mercaptoethanol-treated bovine serum albumin enhances T cell colony formation as does 2-mercaptoethanol. The factor only partially substitutes for FCS, but neither for the mitogen phytohemagglutinin nor for conditioned medium derived from cultures of adherent cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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