Electrical pulse stimulation of cultured skeletal muscle cells as a model forin vitroexercise - possibilities and limitations
| Autor: | Nataša Nikolić, Juergen Eckel, Vigdis Aas, Kristin Eckardt, G. H. Thoresen, Sven W. Görgens |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Stimulation In vitro model 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Muscle Skeletal Exercise Cells Cultured Tissue Engineering Pulse (signal processing) Chemistry Mechanism (biology) Skeletal muscle Anatomy Electric Stimulation In vitro 030104 developmental biology medicine.anatomical_structure medicine.symptom Neuroscience 030217 neurology & neurosurgery Muscle Contraction Muscle contraction |
| Zdroj: | Acta Physiologica. 220:310-331 |
| ISSN: | 1748-1708 |
| Popis: | The beneficial health-related effects of exercise are well recognized, and numerous studies have investigated underlying mechanism using various in vivo and in vitro models. Although electrical pulse stimulation (EPS) for the induction of muscle contraction has been used for quite some time, its application on cultured skeletal muscle cells of animal or human origin as a model of in vitro exercise is a more recent development. In this review, we compare in vivo exercise and in vitro EPS with regard to effects on signalling, expression level and metabolism. We provide a comprehensive overview of different EPS protocols and their applications, discuss technical aspects of this model including critical controls and the importance of a proper maintenance procedure and finally discuss the limitations of the EPS model. |
| Databáze: | OpenAIRE |
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