Long non-coding RNA AL355711 promotes smooth muscle cell migration through the ABCG1/MMP3 pathway
| Autor: | Chun-Min Kang, Jing-Jing Zhao, Xing Jin, Ying-Shi Yuan, Jun Yan, Wei-Ye Chen, Xue-Heng Li, Rui-Ying Huang, Wei-Kang Li, Xianzhang Huang, Peifeng Ke, Ke-Wei Yu, Shunwang Cao, Heng Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Smooth muscle cell migration ATP-binding cassette sub-family G member 1 Mice Knockout ApoE Myocytes Smooth Muscle Type (model theory) Combinatorics Animal model Cell Movement Genetics Animals Humans In patient Cells Cultured ATP Binding Cassette Transporter Subfamily G Member 1 Philosophy General Medicine Articles Atherosclerosis Long non-coding RNA Plaque Atherosclerotic Mice Inbred C57BL Disease Models Animal matrix metalloproteinase 3 Gene Expression Regulation long non-coding RNA AL355711 Correlation analysis Immunohistochemistry RNA Long Noncoding Transcription (software) Vascular smooth muscle cell migration Metabolic Networks and Pathways |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Atherosclerosis and related cardiovascular diseases pose severe threats to human health worldwide. There is evidence to suggest that at least 50% of foam cells in atheromas are derived from vascular smooth muscle cells (VSMCs); the first step in this process involves migration to human atherosclerotic lesions. Long non-coding RNAs (lncRNAs) have been found to play significant roles in diverse biological processes. The present study aimed to investigate the role of lncRNAs in VSMCs. The expression of lncRNAs or mRNAs was detected using reverse transcription-quantitative polymerase chain reaction. The Gene Expression Omnibus datasets in the NCBI portal were searched using the key words 'Atherosclerosis AND tissue AND Homo sapiens' and the {"type":"entrez-geo","attrs":{"text":"GSE12288","term_id":"12288"}}GSE12288 dataset. Gene expression in circulating leukocytes was measured to identify patients with coronary artery disease (CAD) or controls, and used to analyze the correlation coefficient and expression profiles. The protein level of ATP-binding cassette sub-family G member 1 (ABCG1) and matrix metalloproteinase (MMP)3 was determined using immunohistochemistry and western blot analysis. The analysis of mouse aortic roots was performed using Masson's and Oil Red O staining. The expression of lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711, ABCG1 and MMP3 was found to be higher in human atherosclerotic plaques or in patients with atherosclerotic CAD. The correlation analysis revealed that ABCG1 may be involved in the regulation between lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 and MMP3 in atherosclerotic CAD. The knockdown of lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 inhibited ABCG1 transcription and smooth muscle cell migration. In addition, lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 was found to regulate MMP3 expression through the ABCG1 pathway. The expression of ABCG1 and MMP3 was found to be high in an animal model of atherosclerosis. The results indicated that lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 promoted VSMC migration and atherosclerosis partly via the ABCG1/MMP3 pathway. On the whole, the present study demonstrates that the inhibition of lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 may serve as a novel therapeutic target for atherosclerosis. lncRNA {"type":"entrez-nucleotide","attrs":{"text":"AL355711","term_id":"7799156","term_text":"AL355711"}}AL355711 in circulating leukocytes may be a novel biomarker for atherosclerotic CAD. |
| Databáze: | OpenAIRE |
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