Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies

Autor: Alexandre Walmott Borges, Luana Marotta Reis de Vasconcellos, Daphne de Camargo Reis Mello, Artur J.M. Valente, Tabata Sato
Přispěvatelé: Universidade Estadual Paulista (Unesp), CQC
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Polymers
Apoptosis
02 engineering and technology
01 natural sciences
lcsh:Chemistry
Chitosan
chemistry.chemical_compound
Drug Delivery Systems
Anti-Infective Agents
Tumor Cells
Cultured
lcsh:QH301-705.5
Spectroscopy
pectin
Osteosarcoma
Coacervate
Aqueous solution
Hydrogels
General Medicine
021001 nanoscience & nanotechnology
Pectin
Computer Science Applications
propolis
Emulsion
Self-healing hydrogels
Drug delivery
0210 nano-technology
Biocompatibility
Bone Neoplasms
macromolecular substances
010402 general chemistry
complex mixtures
Article
Catalysis
Propolis
Inorganic Chemistry
Humans
Physical and Theoretical Chemistry
Molecular Biology
Cell Proliferation
Organic Chemistry
Aqueous two-phase system
technology
industry
and agriculture
DNA
0104 chemical sciences
Hydrogel
lcsh:Biology (General)
lcsh:QD1-999
chemistry
hydrogel
chitosan
Nuclear chemistry
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 12
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
International Journal of Molecular Sciences, Vol 21, Iss 4561, p 4561 (2020)
Popis: Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µ
g/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm-1, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm&minus
1, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis.
Databáze: OpenAIRE
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