Morphological and Molecular Cytogenetic Characteristics of Giant Human Oocyte
| Autor: | Maryna Petrushko, J. V. Gontar, N. O. Buderatska, Taisiia Yurchuk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pronucleus media_common.quotation_subject Chromosome Embryo molecular cytogenetic analysis Cell Biology Biology 01 natural sciences Agricultural and Biological Sciences (miscellaneous) Andrology 03 medical and health sciences Polar body 030104 developmental biology Human fertilization giant oocytes morphological characteristics Polyploid Meiosis Genetics oocytes Reproduction 010606 plant biology & botany media_common |
| Popis: | The expedience of using oocytes with morphological dysmorphism in assisted reproduction tech- nology programs is debatable. This study aimed to estimate morphological and molecular cytogenetic char- acteristics of enlarged oocytes and to predict results of their further fertilization. Among 774 oocytes retrieved from 80 patients, 83 (11%) cells were enlarged. Morphometric analysis showed the diameter of the ooplasm to be (176.67 ± 1.76) μm and the diameter with ZP (200.8 ± 4.24) μm. Among these giant oocytes, 47 (56.6%) were at the metaphase II stage, 26 (31.3%) at the metaphase I stage, and 10 (12.1%) at the prophase I stage. The retrieved giant oocytes were characterized by dysmorphism of endo- and exocytoplasmic structures. Among 47 cells at the metaphase II stage, 40 (85.1%) oocytes had two polar bodies, three (6.4%) oocytes had one polar body, and four (8.5%) oocytes had a fragmented polar body. The assessment of the presence of mei- otic spindle by polarization microscopy showed that 39 (83%) oocytes had two meiotic spindles, 3 (6.4%) oocytes had one meiotic spindle, and 5 oocytes (10.6%) had no visualized meiotic spindles despite the pres- ence of polar bodies. After fertilization of 47 oocytes at the metaphase II stage, pronuclei were detected in 42 (89.4%) cells, including 9 (21.4%) oocytes with two pronuclei, 27 (63.3%) oocytes with 3 pronuclei, and 6 (15.3%) oocytes with four pronuclei. The molecular cytogenetic analysis demonstrated that the embryos obtained after fertilization of giant oocytes had a polyploid chromosome set number. Thus, the results of our study showed the inexpediency of using giant oocytes for further fertilization. |
| Databáze: | OpenAIRE |
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