Recellularization of a novel off-the-shelf valve following xenogenic implantation into the right ventricular outflow tract

Autor: Jeremy J. Thaden, Jason L. Go, Ryan S. Hennessy, Sorin V. Pislaru, Nicholas J. Stoyles, Robert D. Simari, Amir Lerman, Rebecca R. Hennessy, John M. Stulak, Soumen Jana, Mohammed Al-Hijji, Brandon J. Tefft, Melissa D. Young
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Aortic valve
Male
Pathology
Swine
lcsh:Medicine
Hemodynamics
Blood Pressure
Alizarin Staining
030204 cardiovascular system & hematology
Vascular Medicine
Stiffness
Extracellular matrix
0302 clinical medicine
Medicine and Health Sciences
Group-Specific Staining
Medicine
Ventricular outflow tract
lcsh:Science
Mammals
Staining
Multidisciplinary
Decellularization
Heart
Anatomy
Ruminants
Hematology
Thrombosis
Systolic Pressure
Biomechanical Phenomena
Extracellular Matrix
medicine.anatomical_structure
Phenotype
Echocardiography
Aortic Valve
Heart Valve Prosthesis
Vertebrates
Physical Sciences
Female
Research Article
medicine.medical_specialty
Cell Survival
Heart Ventricles
Materials Science
Material Properties
Transplantation
Heterologous
Research and Analysis Methods
Prosthesis Design
03 medical and health sciences
medicine.artery
Tensile Strength
Animals
Mechanical Properties
Humans
Immunohistochemistry Techniques
Cell Proliferation
Pulmonary Valve
Sheep
Tissue Engineering
business.industry
lcsh:R
Organisms
Biology and Life Sciences
Carbon Dioxide
medicine.disease
Transplantation
Histochemistry and Cytochemistry Techniques
030104 developmental biology
Specimen Preparation and Treatment
Pulmonary artery
Amniotes
Immunologic Techniques
Cardiovascular Anatomy
lcsh:Q
business
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 8, p e0181614 (2017)
ISSN: 1932-6203
Popis: Current research on valvular heart repair has focused on tissue-engineered heart valves (TEHV) because of its potential to grow similarly to native heart valves. Decellularized xenografts are a promising solution; however, host recellularization remains challenging. In this study, decellularized porcine aortic valves were implanted into the right ventricular outflow tract (RVOT) of sheep to investigate recellularization potential. Porcine aortic valves, decellularized with sodium dodecyl sulfate (SDS), were sterilized by supercritical carbon dioxide (scCO2) and implanted into the RVOT of five juvenile polypay sheep for 5 months (n = 5). During implantation, functionality of the valves was assessed by serial echocardiography, blood tests, and right heart pulmonary artery catheterization measurements. The explanted valves were characterized through gross examination, mechanical characterization, and immunohistochemical analysis including cell viability, phenotype, proliferation, and extracellular matrix generation. Gross examination of the valve cusps demonstrated the absence of thrombosis. Bacterial and fungal stains were negative for pathogenic microbes. Immunohistochemical analysis showed the presence of myofibroblast-like cell infiltration with formation of new collagen fibrils and the existence of an endothelial layer at the surface of the explant. Analysis of cell phenotype and morphology showed no lymphoplasmacytic infiltration. Tensile mechanical testing of valve cusps revealed an increase in stiffness while strength was maintained during implantation. The increased tensile stiffness confirms the recellularization of the cusps by collagen synthesizing cells. The current study demonstrated the feasibility of the trans-species implantation of a non-fixed decellularized porcine aortic valve into the RVOT of sheep. The implantation resulted in recellularization of the valve with sufficient hemodynamic function for the 5-month study. Thus, the study supports a potential role for use of a TEHV for the treatment of valve disease in humans.
Databáze: OpenAIRE
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