High Helicobacter pylori Bacterial Load and Low Cytokine Expression Levels Are Associated with Nodular Gastropathy

Autor: Alberto Espino, Carolina Serrano, Rodrigo Mansilla-Vivar, Caroll Hernández, Javiera Torres, Paul R. Harris, Macarena Vera, Arnoldo Riquelme, Margarita Pizarro, Camila Palma, Eduardo Fuentes-López
Rok vydání: 2019
Předmět:
Adult
Male
medicine.medical_specialty
Physiology
medicine.medical_treatment
Interleukin-1beta
Rapid urease test
Gastroenterology
Endosonography
Helicobacter Infections
Narrow Band Imaging
Young Adult
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Internal medicine
Gastric mucosa
medicine
Humans
CagA
Endoscopy
Digestive System
RNA
Messenger
Antigens
Bacterial
Helicobacter pylori
biology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
business.industry
Interleukin-8
Cancer
Middle Aged
Hepatology
biology.organism_classification
medicine.disease
Bacterial Load
medicine.anatomical_structure
Cytokine
Gastric Mucosa
Case-Control Studies
Gastritis
030220 oncology & carcinogenesis
Cytokines
Female
030211 gastroenterology & hepatology
medicine.symptom
business
Zdroj: Digestive Diseases and Sciences. 65:565-575
ISSN: 1573-2568
0163-2116
DOI: 10.1007/s10620-019-05769-2
Popis: Nodular gastropathy (NG) is an inflammatory condition of the gastric mucosa characterized by the endoscopic detection of multiple millimeter protrusions. A strong association between NG and Helicobacter pylori and a possible role of NG as a risk factor for undifferentiated gastric cancer have been described. The aim of this study was to characterize the pathogenic and inflammatory profile of patients with NG. Adult patients referred for upper gastrointestinal endoscopy were prospectively enrolled in this study. H. pylori infection status was determined by rapid urease test. Biopsies were stained with hematoxylin–eosin. Sydney and OLGA scores were used to assess gastritis characteristics and gastric cancer risk. PCR analysis was performed to determine bacterial load and virulence factors CagA (and its EPIYA motifs) and VacA alleles. Finally, gastric mucosa cytokine gene expression (IL-8, IL-1β, and TNF-α) was determined by real-time RT-PCR. Forty-eight patients, mean age of 36 years, were recruited. All NG patients were infected by H. pylori. OLGA score was similar in both groups (NG patients and non-NG patients). NG patients had higher bacterial load in the gastric corpus (p = 0.01) and significantly less pro-inflammatory cytokine levels than non-NG infected patients (p = 0.01). In our study, NG is not associated with preneoplastic lesions. An increase in bacterial load without a concomitant increase in mucosal inflammatory cytokine responses in H. pylori-infected subjects with NG may represent a general dampening of immune responses or an additional mechanism of H. pylori active immune evasion.
Databáze: OpenAIRE
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