Genotoxic effect of epirubicin in mouse bone marrow in vivo
| Autor: | Emin Karakas, Rahmi Bilaloglu, Asuman K. Sen |
|---|---|
| Přispěvatelé: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Uludağ Üniversitesi/Yenişehir Meslek Yüksekokulu., Şen, Asuman Karakaş, Karakaş, Emin, Bilaloğlu, Rahmi, AAI-4483-2021 |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Erythrocytes Bone marrow cell Mouse Biochemistry & molecular biology Pharmacology Sabarubicin Anthracyclines Intraday medicine.disease_cause Median lethal dose Mice Acute promyelocytic leukemia Pathology Breast-cancer Micronucleus Tests Chemistry Lethal dose 50 Erythrocyte medicine.anatomical_structure Damage Micronucleus test Female Epirubicin medicine.drug Bone marrow cells Anthracycline Cells General Biochemistry Genetics and Molecular Biology Article In vivo Anthracycline antibiotics medicine Animals Erythrocyte disorder Pharmacology & pharmacy Animal LD 50 DNA topoisomerase-II Micronucleus induction Drug effect Micronucleus Doxorubicin Erythrocytes abnormal DNA damage Bone marrow Therapy Genotoxicity Cytology |
| Popis: | The genotoxic effect of epirubicin, a semisynthetic anthracycline antibiotic which has been used as an anticancer drug, was investigated in vivo on bone marrow cells of Swiss albino mice using the micronucleus test. To determine the incidence of micronuclei, mice were injected intraperitoneally with the drug at single doses of 4, 6, 8, and 10 mg/kg body weight. Then, bone marrow was sampled 18, 24, 36, and 48 h after the treatment. Polychromatic and normochromatic erythrocytes were examined for the presence of micronuclei. Epirubicin significantly increased the frequency of micronucleated polychromatic erythrocytes (MNPCEs) for all treatment periods compared with the negative control (P < 0.001). The frequency of MNPCEs increased with the dose, but at the highest dose used (which is considered to be quite toxic), the frequency of MNPCEs was rather lower. Epirubicin also decreased the ratio of polychromatic to normochromatic erythrocytes (PCE/NCE) for all sampling intervals, which is indicative of bone marrow cytotoxicity. It can be concluded from the present study that the anticancer drug epirubicin has genotoxic effects on mouse bone marrow cells. |
| Databáze: | OpenAIRE |
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