Tumors in hepatobiliary tract and pancreatic islet tissues of transgenic mice harboring gastrin simian virus 40 large tumor antigen fusion gene
| Autor: | Tatsuzo Oka, Gilbert Jay, Anthony G. Montag, Chel Soon Choi, Kan Agarwal, Kwanghee Baek |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Recombinant Fusion Proteins
Mice Nude Mice Transgenic Simian virus 40 Biology medicine.disease_cause digestive system Fusion gene Mice Antigen Gastrins Pyloric Antrum medicine Gastric mucosa Animals Antigens Viral Tumor Antrum Gastrin Hyperplasia Multidisciplinary Pancreatic islets digestive oral and skin physiology Adenoma Islet Cell Virology Molecular biology Hypoglycemia Biliary Tract Neoplasms medicine.anatomical_structure Gastric Mucosa Carcinogenesis Pancreas hormones hormone substitutes and hormone antagonists Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:6696-6700 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.90.14.6696 |
| Popis: | Gastrin is expressed in the gastric antrum and in fetal pancreatic islets but not in adult islets. We have now identified the hepatobiliary tract as another, previously unknown, potential site of gastrin gene expression. Two human gastrin simian virus 40 large tumor antigen (SV40 T antigen) fusion genes containing 1.5 kb of 5' flanking sequence and 10.5 kb that included 5.5 kb upstream, 1.5 kb downstream, and the entire transcribed region were used to generate transgenic mice. Analysis of several transgenic lines, derived from both fusion genes, revealed development of transmissible hepatobiliary tract tumors and pancreatic islet cell tumors. Analysis of each of the tumor cells demonstrates expression of SV40 T antigen but no expression of gastrin. Of the two fusion genes, only the 10.5-kb sequence induces hyperplasia of gastrin-producing cells in the antrum. Analysis of these cells demonstrates expression of SV40 T antigen and gastrin, suggesting that the 10.5-kb sequence is sufficient for gastrin cell hyperplasia in the antrum. These data raise the possibility that gastrin is transiently expressed in the hepatobiliary tract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|