Production of double-stranded RNA during synthesis of bromouracil-substituted RNA by transcription with T7 RNA polymerase
| Autor: | Christian Cazenave, Frédéric Revers, Tuong Luan Cao |
|---|---|
| Přispěvatelé: | Université Victor Segalen - Bordeaux 2 |
| Jazyk: | angličtina |
| Předmět: |
Bromouracil
Transcription Genetic Molecular Sequence Data 030303 biophysics Biophysics RNA-dependent RNA polymerase Nucleic Acid Denaturation Biochemistry Viral Proteins 03 medical and health sciences Structural Biology Transcription (biology) Genetics medicine RNA polymerase I T7 RNA polymerase RNA synthesis Molecular Biology Polymerase RNA Double-Stranded 030304 developmental biology Base Composition 0303 health sciences Oligoribonucleotides Base Sequence Models Genetic biology Chemistry RNA [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology DNA-Directed RNA Polymerases Cell Biology Molecular biology Transcription (in vitro) RNA editing biology.protein modified nucleotide Small nuclear RNA medicine.drug |
| Zdroj: | FEBS Letters FEBS Letters, Wiley, 1994, 351 (2), pp.253-256. ⟨10.1016/0014-5793(94)00870-1⟩ |
| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/0014-5793(94)00870-1 |
| Popis: | Using T7 RNA polymerase we synthesized a short oligoribonucleotide containing bromouracil by in vitro transcription of a synthetic DNA template. Whereas the major transcript obtained had the expected size and was apparently homogeneous on a sequencing gel, additional analysis revealed the presence of double-stranded RNA in this preparation. As this was not observed when the same template was transcribed in the presence of uracil, we hypothesize that bromouracil promoted the apparition of double-stranded ‘parasitic’ RNA presumably by favouring priming for the RNA-dependent RNA synthesis of the T7 RNA polymerase or by facilitating an end-to-end copy mechanism. |
| Databáze: | OpenAIRE |
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