Nippostrongylus brasiliensis: ability of plasma to prime free radical generation by leukocytes in response to adult worms not due to gamma-interferon or tumour necrosis factor
| Autor: | K.S. Ovington, Nicholas Smith |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Necrosis
Lipopolysaccharide Free Radicals chemistry.chemical_compound Interferon-gamma Immune system In vivo medicine Leukocytes Animals Interferon gamma Nippostrongylus brasiliensis Rats Wistar Strongylida Infections biology Tumor Necrosis Factor-alpha Body Weight Antibodies Monoclonal biology.organism_classification Rats Infectious Diseases Blood chemistry Immunology Luminescent Measurements biology.protein Parasitology Tumor necrosis factor alpha Female Nippostrongylus Antibody medicine.symptom Peritoneum medicine.drug |
| Zdroj: | International journal for parasitology. 24(7) |
| ISSN: | 0020-7519 |
| Popis: | Plasma-borne factors prime leukocytes from both infected and uninfected rats for radical generation in response to N. brasiliensis. The concentration of these factors is increased following infection and reaches maximal levels on day 8 post-infection (p.i.) as demonstrated by the striking ability of plasma from infected rats to prime leukocytes from uninfected rats to produce free radicals in response to adult worms. The cytokines, gamma-interferon and tumour necrosis factor (TNF) can be detected in plasma during infection with a variety of organisms and several lines of immunological and pathophysiological evidence, including radical generation, weight loss, anaemia and diarrhoea, implicate generation of these proteins in response to infection with N. brasiliensis. We therefore investigated whether gamma-interferon and TNF were detectable in the plasma of rats infected with N. brasiliensis and whether the presence of these cytokines correlated with the ability of plasma to enhance radical generation in response to N. brasiliensis. However, gamma-interferon was not detected in the plasma of rats at any time after infection with N. brasiliensis and neutralizing monoclonal antibody to rat gamma-interferon had no effect on the ability of plasma to prime free radical generation. TNF was detected in the plasma of heavily-infected rats but only at very low levels (< 1 ng/ml), though copius in vivo synthesis of TNF could be induced by treatment of the infected rats with lipopolysaccharide (LPS). However, neither parasite-induced nor parasite plus LPS-induced plasma TNF correlated with the ability of plasma to enhance radical generation in response to N. brasiliensis.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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