Chemical composition, antibacterial and anticancer activities of volatile oil ofMelicope denhamiileaves
| Autor: | Sabulal Baby, S. Ajikumaran Nair, Ramaswamy Venkataraman, Sony George |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
food.ingredient
Apoptosis Microbial Sensitivity Tests Plant Science Bacillus subtilis Biology medicine.disease_cause Biochemistry Analytical Chemistry Mice chemistry.chemical_compound food Cell Line Tumor Oils Volatile medicine Animals Plant Oils MTT assay Food science Cytotoxicity Rutaceae Escherichia coli fungi Organic Chemistry food and beverages biology.organism_classification Antineoplastic Agents Phytogenic Anti-Bacterial Agents Plant Leaves chemistry Trypan blue Melicope denhamii Sesquiterpenes Bacteria |
| Zdroj: | Natural Product Research. 29:1959-1962 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2015.1013471 |
| Popis: | Melicope denhamii leaf volatile oil was isolated by hydrodistillation, and twenty-six constituents comprising 95.95% of the leaf oil were characterised by gas chromatographic techniques. Sesquiterpenes, zierone (22.49%) and α-gurjunene (19.96%), were identified as the major components. M. denhamii leaf oil tested against Gram-positive and Gram-negative bacteria showed significant activity against Bacillus subtilis and Escherichia coli. Anticancer activity of M. denhamii leaf oil against Dalton's lymphoma ascites cells was assessed by trypan blue exclusion and MTT assays, and the oil showed significant cytotoxicity at CD50 of 12.2 μg/mL. Induction of apoptosis on DLA cells by M. denhamii leaf oil was confirmed by morphological observation, nuclear damage and comet assays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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