Serum antibodies to West Nile virus in naturally exposed and vaccinated horses
| Autor: | Raymond A. Koski, Louis A. Magnarelli, Michel Ledizet, Sandra L. Bushmich, John F. Anderson |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
viruses Enzyme-Linked Immunosorbent Assay Antibodies Viral Microbiology Virus Neutralization Article Serology Flaviviridae Neutralization Tests biology.animal Animals Horses biology Outbreak virus diseases Viral Vaccines General Medicine biology.organism_classification Virology Flavivirus Immunology biology.protein Horse Diseases Equidae Antibody West Nile virus West Nile Fever |
| Popis: | A polyvalent enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization tests (PRNT) were used to measure serum antibodies to the West Nile virus (WNV) in horses naturally exposed to or vaccinated against this flavivirus in Connecticut and New York State, USA. Relying on a PRNT as a “gold standard”, the main objective was to validate a modified ELISA containing a recombinant WNV envelope protein antigen. It was also important to assess specificity by testing sera from horses that had other, undiagnosed illnesses. Sera for the latter study were obtained from 43 privately owned horses during 1995−1996. Analyses by an ELISA and PRNT confirmed the presence of WNV antibodies in 21 (91%) of 23 sera from naturally exposed horses and in 85% of the 20 vaccinated subjects; overall results for both study groups were highly concordant (91% agreement). Humoral responses of naturally exposed and immunized horses were similar. Both serologic tests were useful in confirming past infections of the WNV, but there was no evidence that horses with undiagnosed illnesses were exposed to the WNV prior to the 1999 outbreak. |
| Databáze: | OpenAIRE |
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