Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO
| Autor: | P V Phibbs, C H MacGregor, J A Wolff, R C Eisenberg |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Mutant
Citric Acid Cycle Catabolite repression Repressor Microbiology Amidohydrolases Bacterial Proteins Transduction Genetic Mannitol Molecular Biology Psychological repression biology Aldolase A Biological Transport Succinates Amides Fed-batch culture Citric acid cycle Repressor Proteins Glucose Biochemistry Genes Bacterial Dehydratase Conjugation Genetic Mutation Pseudomonas aeruginosa biology.protein Research Article |
| Zdroj: | Journal of bacteriology. 173(15) |
| ISSN: | 0021-9193 |
| Popis: | Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in the 11-min region of the P. aeruginosa chromosome near argB and pyrE and were designated crc. Crc- mutants no longer showed repression of mannitol and glucose transport, glucose-6-phosphate dehydrogenase, glucokinase, Entner-Doudoroff dehydratase and aldolase, and amidase when grown in the presence of succinate plus an inducer. These activities were not expressed constitutively in Crc- mutants but exhibited wild-type inducible expression. |
| Databáze: | OpenAIRE |
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